{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T14:55:12Z","timestamp":1780325712347,"version":"3.54.1"},"reference-count":30,"publisher":"Wiley","issue":"24","license":[{"start":{"date-parts":[[2024,10,12]],"date-time":"2024-10-12T00:00:00Z","timestamp":1728691200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100019305","name":"Manipal Academy of Higher Education","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100019305","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2024,12,30]]},"abstract":"<jats:sec>\n                    <jats:title>Rationale<\/jats:title>\n                    <jats:p>The exploration of the gut microbiome and related metabolites holds an exciting future in health science. The challenges associated with fecal sample testing are proper sample collection, sterile transportation, optimal transport conditions, and processing as all these factors could potentially change the microbiome composition, further exacerbated by the patient's customary discomfort regarding feces samples. The study aimed to compare the usage of rectal swabs and stool samples for short\u2010chain fatty acid estimation using gas chromatography\u2013mass spectrometry (GC\u2013MS) and indole estimation using spectrophotometry.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Method<\/jats:title>\n                    <jats:p>From May 2022 to June 2022, three women were recruited from the Department of Obstetrics and Gynecology (OBG) in a secondary care hospital in coastal Karnataka. During their clinical visit, a rectal swab was collected, and the stool sample was transported to the hospital from the patient's home in sterile containers provided. After the extraction, short\u2010chain fatty acids (acetate, propionate, and butyrate) were quantified using GC\u2013MS. The fecal indole concentration was determined using a hydroxylamine\u2010based assay.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The GC\u2013MS analysis failed to detect the concentrations of short\u2010chain fatty acids in rectal swab samples. Indole concentrations in stool and swab samples were significantly different.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The study's findings do not support the use of rectal swabs to analyze gut metabolites.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.9923","type":"journal-article","created":{"date-parts":[[2024,10,12]],"date-time":"2024-10-12T06:28:32Z","timestamp":1728714512000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["GC\u2013MS quantification of fecal short\u2010chain fatty acids and spectrophotometric detection of indole: Do rectal swabs produce comparable results as stool samples? \u2010 A pilot study"],"prefix":"10.1002","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6635-6339","authenticated-orcid":false,"given":"Vineetha","family":"K K","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology, Melaka Manipal Medical College Manipal Academy of Higher Education  Manipal Karnataka India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Archana","family":"P R","sequence":"additional","affiliation":[{"name":"Department of Basic Medical Sciences Manipal Academy of Higher Education  Manipal Karnataka India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bhamini Krishna","family":"Rao","sequence":"additional","affiliation":[{"name":"Department of Physiotherapy, Manipal College of Health Professions Manipal Academy of Higher Education  Manipal Karnataka India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mamatha","family":"Ballal","sequence":"additional","affiliation":[{"name":"Division of Enteric Diseases and Department of Microbiology, Kasturba Medical College Manipal Academy of Higher Education  Manipal Karnataka 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