{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T20:52:53Z","timestamp":1790801573551,"version":"4.1.0"},"reference-count":38,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,10,22]],"date-time":"2024-10-22T00:00:00Z","timestamp":1729555200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003246","name":"Nederlandse Organisatie voor Wetenschappelijk Onderzoek","doi-asserted-by":"publisher","award":["Vici 16131"],"award-info":[{"award-number":["Vici 16131"]}],"id":[{"id":"10.13039\/501100003246","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2025,1,15]]},"abstract":"<jats:p>\n                    Mass spectrometry imaging (MSI) is a powerful tool for detecting lipids in tissue sections, with matrix\u2010assisted laser desorption\/ionization (MALDI) and desorption electrospray ionization (DESI) as its key ionization techniques. In this study, we examine how MALDI compares with state\u2010of\u2010the\u2010art DESI ionization in identifying lipids in heterogeneous samples, specifically atherosclerotic plaques. Carotid plaques (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u20094) from patients undergoing endarterectomy were snap\u2010frozen, stored at \u221280\u00b0C, and then sectioned for MSI analysis and H&amp;E staining. Measurements were conducted using a SYNAPT XS mass spectrometer in positive ion mode, employing MALDI with a 2,5\u2010dihydroxybenzoic acid (DHB) matrix and DESI with a methanol: water (\n                    <jats:italic>98:2<\/jats:italic>\n                    ) (\n                    <jats:italic>v\/v<\/jats:italic>\n                    ) solvent. Our comparison covered spectral profiles, sensitivity, and image quality generated by these two techniques. We found that both MALDI and DESI are highly suitable techniques for detecting a wide range of lipids in atherosclerotic plaque sections. DESI\u2010MSI exhibited higher ion counts for most lipid classes than MALDI\u2010MSI and provided sharper images. MALDI detected larger amounts of ceramide and hexosylceramide species, possibly due to its efficient generation of dehydrated ions. In contrast, DESI showed greater peak intensities of cholesteryl ester and triacylglyceride species than MALDI, consistent with reduced fragmentation. These findings establish the relative merits of DESI and MALDI and demonstrate their complementarity as techniques for lipid research in MSI.\n                  <\/jats:p>","DOI":"10.1002\/rcm.9927","type":"journal-article","created":{"date-parts":[[2024,10,22]],"date-time":"2024-10-22T06:46:03Z","timestamp":1729579563000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["MALDI versus DESI mass spectrometry imaging of lipids in atherosclerotic plaque"],"prefix":"10.1002","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6922-2045","authenticated-orcid":false,"given":"Nuria","family":"Slijkhuis","sequence":"first","affiliation":[{"name":"Department of Cardiology Cardiovascular Institute, Thorax Center, Erasmus MC  Rotterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark","family":"Towers","sequence":"additional","affiliation":[{"name":"Waters Corporation  Wilmslow UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emmanuelle","family":"Claude","sequence":"additional","affiliation":[{"name":"Waters Corporation  Wilmslow UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gijs","family":"van Soest","sequence":"additional","affiliation":[{"name":"Department of Cardiology Cardiovascular Institute, Thorax Center, Erasmus MC  Rotterdam The Netherlands"},{"name":"Department of Precision and Microsystems Engineering Delft University of Technology  Delft The Netherlands"},{"name":"Wellman Center for Photomedicine Massachusetts General Hospital  Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,10,22]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40169\u2010018\u20100190\u20109"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978\u20100\u201012\u2010800177\u20106.00001\u20103"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr100012c"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth1094"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr3004295"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr200280p"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2018.01238"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2016.02.014"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1104404"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbalip.2011.05.006"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/jms.922"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2008.12.058"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1039\/b925257f"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/978\u20101\u20100716\u20103319\u20109_4"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talo.2021.100046"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199901143400207"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.RA120000974"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/jasms.0c00070"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.atherosclerosis.2011.03.044"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.3390\/metabo11040250"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jlr.2024.100504"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac901739s"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.atherosclerosis.2022.11.019"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.atherosclerosis.2023.117340"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijms.2018.09.015"},{"key":"e_1_2_7_27_1","unstructured":"TowersM JonesE BallantyneJ JarvisS eds.High resolution low flow DESI imaging using a commercial DESI source. 72nd ASMS conference on mass spectrometry and allied topics.2024; Anaheim California."},{"key":"e_1_2_7_28_1","unstructured":"JonesEA HoyesE TrinkleS ChapmanR eds.DESI imaging at the cellular level through the application of nano\u2010flow and multi\u2010focus approaches 71st ASMS conference on mass spectrometry and allied topics.2023."},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13361\u2010019\u201002254\u2010y"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.3444"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.1c02826"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.858"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2022.891018"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac5028534"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac2033547"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.atherosclerosis.2015.10.022"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.116.305675"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvs368"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.12997\/jla.2021.10.3.268"}],"container-title":["Rapid Communications in Mass Spectrometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/rcm.9927","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T14:52:54Z","timestamp":1761490374000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/rcm.9927"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10,22]]},"references-count":38,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,1,15]]}},"alternative-id":["10.1002\/rcm.9927"],"URL":"https:\/\/doi.org\/10.1002\/rcm.9927","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1002\/RCM.9927\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/RCM.9927\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/RCM.9927\/v1\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/RCM.9927\/v2\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/RCM.9927\/v1\/decision1","asserted-by":"object"}]},"ISSN":["0951-4198","1097-0231"],"issn-type":[{"value":"0951-4198","type":"print"},{"value":"1097-0231","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,10,22]]},"assertion":[{"value":"2024-07-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-10-02","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-10-22","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e9927"}}