{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,13]],"date-time":"2026-06-13T09:39:35Z","timestamp":1781343575175,"version":"3.54.1"},"reference-count":17,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,11,12]],"date-time":"2024-11-12T00:00:00Z","timestamp":1731369600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2025,1,30]]},"abstract":"\n Background<\/jats:title>\n Therapeutic drug monitoring is an integral part of organ transplantation. A rapid, simple, economical, and robust high\u2010performance liquid chromatography\u2010tandem mass spectrometry (HPLC\u2010MS\/MS) method for simultaneously determining the immunosuppressants cyclosporine A and tacrolimus might increase detection efficiency.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n In this study, we developed and validated a rapid HPLC\u2010MS\/MS method. Whole blood samples of 100\u2009\u03bcL were prepared by protein precipitation with acetonitrile and 0.5\u00a0mol. L\n \u22121<\/jats:sup>\n ZnSO\n 4<\/jats:sub>\n . Chromatography was performed on a pre\u2010column using a gradient elution with 20\u00a0mmol. L\n \u22121<\/jats:sup>\n ammonium formate and 0.1% (v\/v) formic acid in water (mobile phase A) and 0.1% (v\/v) formic acid in methanol (mobile phase B) at a flow rate of 1.5\u00a0mL.min\n \u22121<\/jats:sup>\n . The analysis time was 2.2\u00a0min. Electrospray ionization and multiple reaction monitoring were performed. The lower limit of quantification was set at 1\u00a0ng. L\n \u22121<\/jats:sup>\n for tacrolimus and 50\u2009ng. L\n \u22121<\/jats:sup>\n for cyclosporine A.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n The method showed adequate accuracy and precision with a sufficient linear range. The calibration curve range of tacrolimus and cyclosporine A was 1\u201330 and 50\u20131500\u2009ng\u00b7mL\u2010\n 1<\/jats:sup>\n , respectively. All correlation coefficients were >0.99.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n The developed HPLC\u2010MS\/MS is rapid and can be used for simultaneous monitoring of tacrolimus and cyclosporine A.<\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/rcm.9932","type":"journal-article","created":{"date-parts":[[2024,11,12]],"date-time":"2024-11-12T04:20:02Z","timestamp":1731385202000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Development and validation of a rapid HPLC\u2010MS\/MS method for simultaneous determination of cyclosporine A and tacrolimus in whole blood for routine therapeutic drug monitoring in organ transplantation"],"prefix":"10.1002","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9577-7298","authenticated-orcid":false,"given":"Fei\u2010fei","family":"Han","sequence":"first","affiliation":[{"name":"Beijing Chao\u2010Yang Hospital Capital Medical University Beijing 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