{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T22:18:15Z","timestamp":1780525095524,"version":"3.54.1"},"reference-count":29,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,11,26]],"date-time":"2024-11-26T00:00:00Z","timestamp":1732579200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001711","name":"Schweizerischer Nationalfonds zur F\u00f6rderung der Wissenschaftlichen Forschung","doi-asserted-by":"publisher","award":["P500PN_206731"],"award-info":[{"award-number":["P500PN_206731"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004189","name":"Max-Planck-Gesellschaft","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004189","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2025,2,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Rationale<\/jats:title>\n                    <jats:p>\n                      Compound\u2010specific stable isotope analysis of hydrogen (\u03b4D) and carbon (\u03b4\n                      <jats:sup>13<\/jats:sup>\n                      C) in organic compounds is a valuable tool in biogeochemical research. A key limitation of this method is the relatively large amount of sample required to achieve desirable precision.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      We developed a large\u2010volume (20\u2009\u03bcL) injection method that allows for high throughput analysis of less concentrated samples and tested it for \u03b4\n                      <jats:sup>13<\/jats:sup>\n                      C and \u03b4D measurements of\n                      <jats:italic>n<\/jats:italic>\n                      \u2010alkanes. We also conducted a comparison of reference standards and assessed several methods to normalize and correct\n                      <jats:italic>n<\/jats:italic>\n                      \u2010alkane \u03b4D and \u03b413C measurements.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The mean precision of the \u03b4D method based on 233 environmental\n                      <jats:italic>n<\/jats:italic>\n                      \u2010alkane samples (two to three replications per sample) is 4.0\u2030 (1\u03c3, estimated from the weighted mean of the pooled unbiased standard deviations) and 0.46\u2030 (1\u03c3) for \u03b4\n                      <jats:sup>13<\/jats:sup>\n                      C from 37 environmental samples (two to three replications per sample). The evaluation of reference standards shows that the use of\n                      <jats:italic>n<\/jats:italic>\n                      \u2010alkane standards with large offsets in \u03b4D values in adjacent\n                      <jats:italic>n<\/jats:italic>\n                      \u2010alkane chains can lead to biases in measurement correction.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      The large\u2010volume injection method shows good reproducibility of \u03b4\n                      <jats:sup>13<\/jats:sup>\n                      C and \u03b4D measurements of\n                      <jats:italic>n<\/jats:italic>\n                      \u2010alkanes and reduces the required sample concentration by about 80%. We propose that for \u03b4D measurements, a reference standard set should be used in which each reference standard has a limited range of \u03b4D values and no adjacent\n                      <jats:italic>n<\/jats:italic>\n                      \u2010alkane chains, to minimize memory effects.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.9943","type":"journal-article","created":{"date-parts":[[2024,11,26]],"date-time":"2024-11-26T05:54:57Z","timestamp":1732600497000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Large\u2010Volume Injection and Assessment of Reference Standards for\n                    <i>n<\/i>\n                    \u2010Alkane \u03b4D and \u03b4\n                    <sup>13<\/sup>\n                    C Analysis via Gas Chromatography Isotope Ratio Mass Spectrometry"],"prefix":"10.1002","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7340-4768","authenticated-orcid":false,"given":"Paul\u00a0D.","family":"Zander","sequence":"first","affiliation":[{"name":"Climate Geochemistry Department Max Planck Institute for Chemistry  Mainz Germany"},{"name":"Center for Accelerator Mass Spectrometry Lawrence Livermore National Laboratory  Livermore California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florian","family":"Rubach","sequence":"additional","affiliation":[{"name":"Climate Geochemistry Department Max Planck Institute for Chemistry  Mainz Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alfredo","family":"Mart\u00ednez\u2010Garc\u00eda","sequence":"additional","affiliation":[{"name":"Climate Geochemistry Department Max Planck Institute for Chemistry  Mainz Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,11,26]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.palaeo.2013.01.004"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1060143"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41561\u2010019\u20100399\u20102"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev\u2010earth\u2010042711\u2010105535"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature03945"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1160485"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0146\u20106380(90)90147\u2010R"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac50033a022"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac980248v"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.200600002"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098\u20102787(1997)16:5<227::AID\u2010MAS1>3.0.CO;2\u2010J"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.8084"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gloplacha.2016.03.011"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41561\u2010021\u201000786\u20101"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.orggeochem.2020.103979"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.5194\/cp\u201020\u2010841\u20102024"},{"key":"e_1_2_8_18_1","volume-title":"R: A Language and Environment for Statistical Computing","author":"R Core Team","year":"2023"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac000489e"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1467\u20109868.2010.00749.x"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1201\/9781315370279"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.2307\/1418879"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gca.2013.12.021"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac801170v"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.6120"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.3817"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.1009"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.3342"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.quascirev.2013.01.007"},{"key":"e_1_2_8_30_1","volume-title":"Data and Code for \u201cLarge Volume Injection and Assessment of Reference Standards for n\u2010Alkane \u03b4D and \u03b413C Analysis via gas Chromatography Isotope Ratio Mass Spectrometry\u201d [Data set]","author":"Zander P. 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