{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T05:56:56Z","timestamp":1773381416959,"version":"3.50.1"},"reference-count":66,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,12,2]],"date-time":"2024-12-02T00:00:00Z","timestamp":1733097600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2025,2,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Cupressus torulosa<\/jats:italic>\n                      (family Cupressaceae), widely distributed in the northwestern Himalayan region of India, is a coniferous aromatic tree traditionally used for its aerial parts. Its needles are renowned for their anti\u2010inflammatory, anticonvulsant, antimicrobial, and wound\u2010healing properties. Antioxidants from this plant are vital in combating oxidative stress, which are implicated in these diseases.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      This study employed a range of methods, including in vitro, in vivo, and ex vivo assays; biochemical estimations such as Total Phenolics Content and Total Flavonoids Content; and UPLC\u2010QTOF\u2010MS analysis, to evaluate the antioxidant potential of\n                      <jats:italic>Cupressus torulosa<\/jats:italic>\n                      needles.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Initial screenings demonstrated promising antioxidant activity of 25% aqueous methanol extract of the needles, with SGOT and SGPT values of 55.28\u00b157.07 and 52.27\u00b119.50 IU\/L, respectively, when compared to the positive control glibenclamide. Fractionation of the extract into ethyl acetate and butanol fractions revealed that the ethyl acetate fraction showed notable activity, with an EC50 value of 85.6 \u03bcg\/mL in the DPPH assay, indicating effective free radical scavenging and reduced oxidative damage. UPLC\u2010QTOF\u2010MS characterization of ethyl acetate fraction in negative ion mode identified 34 metabolites, including 10 key antioxidant compounds.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      These findings underscore\n                      <jats:italic>C. torulosa\u2019s<\/jats:italic>\n                      potential as a source of bioactive antioxidants, highlighting its value for pharmaceutical and nutraceutical applications. The research provides a foundation for further isolation, characterization, and clinical testing of these compounds, enhancing our understanding of natural antioxidants in addressing oxidative stress\u2010related conditions.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.9945","type":"journal-article","created":{"date-parts":[[2024,12,2]],"date-time":"2024-12-02T23:08:57Z","timestamp":1733180937000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["<i>Cupressus torulosa<\/i>\n                    , a Source of Antioxidant Polyphenols: An Analytical and Pharmacological Study"],"prefix":"10.1002","volume":"39","author":[{"given":"Radhika","family":"Khanna","sequence":"first","affiliation":[{"name":"Chemistry &amp; Bioprospecting Division Forest Research Institute  Dehradun India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.\u00a0R.","family":"Chitme","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy Dehradun Institute of Technology University  Dehradun India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Khushaboo","family":"Bhadoriya","sequence":"additional","affiliation":[{"name":"Chemistry &amp; Bioprospecting Division Forest Research Institute  Dehradun India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V.\u00a0K.","family":"Varshney","sequence":"additional","affiliation":[{"name":"Chemistry &amp; Bioprospecting Division Forest Research Institute  Dehradun India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,12,2]]},"reference":[{"key":"e_1_2_11_2_1","first-page":"156","article-title":"Free Radical Related Effects and Antioxidants in Saliva and Serum of Adolescents With Type 1 Diabetes Mellitus","volume":"40","author":"Nagler R.","year":"2006","journal-title":"Archives of Oral Bioliogy"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.2174\/1381612043384655"},{"key":"e_1_2_11_4_1","first-page":"794","article-title":"Review: Free Radicals and Antioxidants in Human Health: Current Status and Future Prospects","volume":"52","author":"Devasagayam T. P. A.","year":"2004","journal-title":"Journal of Association of Physicians of India"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2221-1691(12)60061-6"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00217-004-1012-4"},{"key":"e_1_2_11_7_1","doi-asserted-by":"crossref","unstructured":"L. K.MacDonald\u2010Wicks L. G.Wood andM. L.Garg \u201cMethodology for the Determination of Biological Antioxidant Capacity in vitro: A Review \u201d \u201cJournal of the Science of Food and Agriculture \u201d (2006) 86 2046.","DOI":"10.1002\/jsfa.2603"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/ffj.1961"},{"issue":"01","key":"e_1_2_11_9_1","first-page":"17","article-title":"Antioxidant Activity of Herbal Plants: A Recent Review","volume":"1","author":"Patel Tyagi J.","year":"2013","journal-title":"Journal of Drug Discovery and Therapeutics"},{"key":"e_1_2_11_10_1","first-page":"1158","article-title":"Nepal Fl Cupressus torulosa, Himalayan Cypress","volume":"699","author":"Prodr N.","year":"1825","journal-title":"Trees of Great Britain and Ireland Cupressus"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.4103\/0250\u2010474X.106078"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2023.116578"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0023\u20106438(95)80008\u20105"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1006\/abbi.1994.1485"},{"key":"e_1_2_11_15_1","first-page":"1217","article-title":"Comparative Study of Antioxidant, Metal Chelating and Antiglycation Activities of Momordica Charantia Flesh and Pulp Fractions","volume":"28","author":"Ghous T.","year":"2015","journal-title":"Pakistan Journal of Pharmaceutical Sciences"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.5264\/eiyogakuzashi.44.307"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/10.6.1003"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox4020394"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.2147\/JEP.S249964"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12906\u2010015\u20100779\u20100"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules15064478"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/18.11.1442b"},{"key":"e_1_2_11_23_1","volume-title":"Fundamentals of Clinical Chemistry","author":"Tietz N.","year":"1986"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/18.6.567"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/26.5.551"},{"key":"e_1_2_11_26_1","first-page":"551","article-title":"Serum Creatinine Determination Without Protein Precipitation","volume":"37","author":"Bartel H.","year":"1972","journal-title":"Clinica Chimica Acta"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.3109\/00365516509077065"},{"key":"e_1_2_11_28_1","first-page":"1245","volume-title":"Textbook of Clinical Chemistry","author":"Tietz N. W.","year":"1999"},{"key":"e_1_2_11_29_1","doi-asserted-by":"crossref","unstructured":"T. O.Tiffany J.Jansen C. A.Burtis J. B.Overton andC.Scott \u201cEnzymatic Kinetic Rate and End\u2010Point Analyses of Substrate by Use of a GeMSAEC Fast Analyzer \u201d \u201cClinical Chemistry \u201d18(1972):829\u2013840.","DOI":"10.1093\/clinchem\/18.8.829"},{"key":"e_1_2_11_30_1","doi-asserted-by":"crossref","unstructured":"H.TakeandG. E.Schubert \u201cEnzymatische Harnstoffbestimmung in Blut und Serum im Optischen Test Nach Warburg [Enzymatic Urea Determination in the Blood and Serum in the Warburg Optical Test] \u201d \u201cKlinische Wochenschrift 43(1965):174 https:\/\/doi.org\/10.1007\/BF01484513.","DOI":"10.1007\/BF01484513"},{"key":"e_1_2_11_31_1","doi-asserted-by":"crossref","unstructured":"D. S.Young \u201cEffects of drugs on Clinical laboratory tests \u201d Third Edition 21(1990):5.","DOI":"10.1016\/0306-3623(90)91072-Y"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules27217625"},{"key":"e_1_2_11_33_1","first-page":"R1","article-title":"Prostaglandin Synthetase Inhibiting O2 Radical Scavenging Properties of Some Flavonoids and Related Phenolic Compounds. Deutsche Pharmakologische Gesellschaft Abstracts of the 20th Spring Meeting, Naunyn\u2010Schmiedebergs Abstract no: R27 Cited","volume":"307","author":"Baumann J.","year":"1979","journal-title":"Archiv der Pharmazie \u2013 Chemistry in Life Sciences"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2009.01.091"},{"key":"e_1_2_11_35_1","unstructured":"A.RoyandT.Sitalakshmi \u201cIn Vitro Antioxidant and Free Radical Scavenging Activity of the Ethanolic extract ofAesculus hippocastanum \u201d (2013)5 403\u2013407."},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/19.1.92"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-91-22330"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/0163-7258(89)90050-8"},{"key":"e_1_2_11_39_1","first-page":"106","article-title":"Lipid Peroxidation Products and Antioxidants in Human Disease","volume":"5","author":"Romero F. J.","year":"1998","journal-title":"Environmental Health Perspectives"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.21010\/ajtcam.v13i5.15"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0741-8329(02)00215-X"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00217-010-1291-x"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jss.2009.03.022"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1093\/qjmed\/hcl092"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.4628"},{"key":"e_1_2_11_46_1","first-page":"142","article-title":"Protective Effect of Thymoquinone Against Morphine Injuries to Kidneys of Mice","volume":"11","author":"Jalili C.","year":"2017","journal-title":"Iranian Journal of Kidney Diseases"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0308-8146(02)00186-3"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2017.08.117"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.33263\/BRIAC125.59185940"},{"key":"e_1_2_11_50_1","doi-asserted-by":"publisher","DOI":"10.3390\/MOLECULES25245850"},{"key":"e_1_2_11_51_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/849769"},{"key":"e_1_2_11_52_1","doi-asserted-by":"publisher","DOI":"10.1055\/s\u20100034\u20101383211"},{"key":"e_1_2_11_53_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13659\u2010021\u201000298\u2010w"},{"key":"e_1_2_11_54_1","doi-asserted-by":"publisher","DOI":"10.1039\/c5ra01911g"},{"key":"e_1_2_11_55_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules25133088"},{"key":"e_1_2_11_56_1","doi-asserted-by":"publisher","DOI":"10.1177\/1934578X19874174"},{"key":"e_1_2_11_57_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu14214500"},{"key":"e_1_2_11_58_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/181260"},{"key":"e_1_2_11_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0028\u20103908(03)00068\u20106"},{"key":"e_1_2_11_60_1","doi-asserted-by":"publisher","DOI":"10.1186\/1475\u20102891\u201013\u201063"},{"key":"e_1_2_11_61_1","doi-asserted-by":"publisher","DOI":"10.14260\/jemds\/2020\/536"},{"key":"e_1_2_11_62_1","doi-asserted-by":"publisher","DOI":"10.3390\/foods10123152"},{"key":"e_1_2_11_63_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf901053x"},{"key":"e_1_2_11_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-3542(98)00033-3"},{"key":"e_1_2_11_65_1","doi-asserted-by":"publisher","DOI":"10.1002\/ddr.21417"},{"key":"e_1_2_11_66_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2009.09.005"},{"key":"e_1_2_11_67_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2021.768708"}],"container-title":["Rapid Communications in Mass Spectrometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/rcm.9945","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T15:02:00Z","timestamp":1761490920000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/rcm.9945"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12,2]]},"references-count":66,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2025,2,15]]}},"alternative-id":["10.1002\/rcm.9945"],"URL":"https:\/\/doi.org\/10.1002\/rcm.9945","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1002\/RCM.9945\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/RCM.9945\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/RCM.9945\/v2\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/RCM.9945\/v4\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/RCM.9945\/v3\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/RCM.9945\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/RCM.9945\/v3\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/RCM.9945\/v3\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/RCM.9945\/v4\/decision1","asserted-by":"object"}]},"ISSN":["0951-4198","1097-0231"],"issn-type":[{"value":"0951-4198","type":"print"},{"value":"1097-0231","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,12,2]]},"assertion":[{"value":"2024-07-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-11-04","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-12-02","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e9945"}}