{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T15:16:02Z","timestamp":1772118962500,"version":"3.50.1"},"reference-count":35,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,12,2]],"date-time":"2024-12-02T00:00:00Z","timestamp":1733097600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2025,2,28]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Rationale<\/jats:title>\n                    <jats:p>Sanguisorbae Radix that mainly contains tannins and phenolic compounds has been widely used as a traditional Chinese medicine for treating hemafecia, hemorrhoids metrorrhagia and metrostaxis in clinics. However, there is no report about the sulfate phenolic compounds in Sanguisorbae Radix.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Extraction of Sanguisorbae Radix was separated and purified by polyamide resin and octadecyl silane\u2010bonded silica, which were analyzed by HPLC\u2010IT\u2010TOF\/MS. Analysis of rat plasma and urine samples using UPLC\u2010Q\u2010TOF\/MS. Finally, the newly discovered sulfate esters in Sanguisorbae Radix were investigated by molecular docking to find out the linkage and the possible mechanism to psoriasis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The chemical structures of methyl\u20103\u2010O\u2010methylgallate\u20105\u2010O\u2010sulfate and methyl\u20103\u2010O\u2010methylgallate were elucidated by the spectral data of HRESIMS and NMR. In addition, 6 sulfate compounds were rapidly identified in Sanguisorbae Radix for the first time, among which 3 sulfate compounds were new natural products. Sixteen prototypes and 11 metabolites were found in rat plasma and urine samples, including 6 sulfate phenolic compounds, and their metabolic pathways in\u00a0vivo were inferred. Molecular docking technique was used to validate the binding activity of methyl\u20103\u2010O\u2010methylgallate\u20105\u2010O\u2010sulfate to psoriasis\u2010related target proteins.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>In this study, sulfate phenolic compounds with high content in Sanguisorbae Radix and biological samples were isolated and characterized by modern spectroscopic techniques, which may provide a theoretical basis for the study of the pharmacological activity and quality control of Sanguisorbae Radix and development of sulfate drugs with a similar structure.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.9947","type":"journal-article","created":{"date-parts":[[2024,12,2]],"date-time":"2024-12-02T23:39:20Z","timestamp":1733182760000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Analysis of Sulfate Compounds From Sanguisorbae Radix and Its Major Metabolites in Rats by UPLC\u2010MS\/MS and Molecular Docking"],"prefix":"10.1002","volume":"39","author":[{"given":"Qing","family":"Wang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Chinese Medicine Modernization Tasly Pharmaceutical Group Co. Ltd.  Tianjin China"},{"name":"Tianjin Key Laboratory of Component\u2010Based Chinese Medicine Tasly Pharmaceutical Group Co. Ltd.  Tianjin China"},{"name":"School of Pharmaceutical Science and Technology Tianjin University  Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhipeng","family":"Huo","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Chinese Medicine Modernization Tasly Pharmaceutical Group Co. Ltd.  Tianjin China"},{"name":"Tianjin Key Laboratory of Component\u2010Based Chinese Medicine Tasly Pharmaceutical Group Co. Ltd.  Tianjin China"},{"name":"School of Chinese Materia Medica Tianjin University of Traditional Chinese Medicine  Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-5419-4732","authenticated-orcid":false,"given":"Bingjing","family":"Han","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Chinese Medicine Modernization Tasly Pharmaceutical Group Co. Ltd.  Tianjin China"},{"name":"Tianjin Key Laboratory of Component\u2010Based Chinese Medicine Tasly Pharmaceutical Group Co. Ltd.  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