{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T15:16:02Z","timestamp":1772118962510,"version":"3.50.1"},"reference-count":20,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2024,12,30]],"date-time":"2024-12-30T00:00:00Z","timestamp":1735516800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100012247","name":"Program of Shanghai Academic Research Leader","doi-asserted-by":"publisher","award":["22XD1420900"],"award-info":[{"award-number":["22XD1420900"]}],"id":[{"id":"10.13039\/501100012247","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32171434"],"award-info":[{"award-number":["32171434"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2025,4,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Rationale<\/jats:title>\n                    <jats:p>The performance of the capillary column directly impacts the separation efficiency of complex sample in liquid chromatography\u2013mass spectrometry\u2013based proteomics studies. The hydraulic packing system offers an effective solution by reducing packing time and expediting the preparation process of column preparation. However, its operational complexity and strict parameter regulation requirements hinder efficient application.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>This study developed an innovative device for the hydraulic capillary column packing, followed by a systematic optimization and evaluation of crucial parameters such as packing pressure, magnetic stirring rate, concentration of solid\u2010phase material, and choice of packing solvent in the capillary column packing process.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The comprehensive evaluation of the prepared capillary columns showed that the hydraulic packing method enabled rapid and large\u2010scale preparation, while also demonstrating exceptional stability and reproducibility.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The proposed hydraulic capillary column packing strategy in this study holds theoretical and practical implications for advancing the technology of column preparation, enhancing separation efficiency, and expanding the depth of analysis in proteomics research.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.9966","type":"journal-article","created":{"date-parts":[[2024,12,30]],"date-time":"2024-12-30T07:20:53Z","timestamp":1735543253000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Comprehensive Optimization of Packing Parameters for Hydraulic\u2010Packed Capillary Columns"],"prefix":"10.1002","volume":"39","author":[{"given":"Chunqiu","family":"Hu","sequence":"first","affiliation":[{"name":"Jiangsu Key Laboratory of Marine Pharmaceutical Compound Screening, College of Pharmacy Jiangsu Ocean University  Lianyungang China"},{"name":"Translational Research Institute of Brain and Brain\u2010Like Intelligence, Shanghai Fourth People's Hospital, and Cancer Center, School of Medicine Tongji University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hang","family":"Fu","sequence":"additional","affiliation":[{"name":"New School of Chinese Materia Medica Nanjing University of Chinese Medicine  Nanjing China"},{"name":"State Key Laboratory of Drug Research Shanghai Institute of Materia Medica, Chinese Academy of Sciences  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lulu","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Drug Research Shanghai Institute of Materia Medica, Chinese Academy of Sciences  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuan","family":"Gao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Drug Research Shanghai Institute of Materia Medica, Chinese Academy of Sciences  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruidong","family":"Li","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Marine Pharmaceutical Compound Screening, College of Pharmacy Jiangsu Ocean University  Lianyungang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhen","family":"Li","sequence":"additional","affiliation":[{"name":"Shanghai Easymass Co., Ltd.  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuai","family":"Li","sequence":"additional","affiliation":[{"name":"Shanghai Easymass Co., Ltd.  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaowang","family":"Xie","sequence":"additional","affiliation":[{"name":"Shanghai Easymass Co., Ltd.  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chongxi","family":"Ren","sequence":"additional","affiliation":[{"name":"Shanghai Easymass Co., Ltd.  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fang","family":"Guo","sequence":"additional","affiliation":[{"name":"Shanghai Easymass Co., Ltd.  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