{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T23:55:39Z","timestamp":1773100539714,"version":"3.50.1"},"reference-count":37,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,12,26]],"date-time":"2024-12-26T00:00:00Z","timestamp":1735171200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2025,3,30]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Rationale<\/jats:title>\n                    <jats:p>Matrix\u2010assisted laser desorption ionization time\u2010of\u2010flight mass spectrometry (MALDI\u2010TOF\u2010MS) is a powerful method for identifying viruses via nucleic acid detection. The data processing method is critical in recognizing nucleic acid obtained by MALDI\u2010TOF\u2010MS. Therefore, new development of data algorithm is needed for virus identification.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>In this work, we developed a new data processing algorithm of MALDI\u2010TOF\u2010MS to identify respiratory viruses and deafness gene mutation sites. The algorithm includes denoising, baseline correcting, peak identification, and extraction features for processing the MS spectrum.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Standard nucleic acid and respiratory virus samples were used to evaluate the performances of the newly developed algorithms. The errors of peak detection were found to be less than 200\u2009ppm. Excellent sensitivity (91.67%\u2013100%) and specificity (96.88\u2013100%) were obtained by identifying 305 virus samples in this work, showing excellent performances. Additionally, accurate identification of the mutation sites of deafness genes was also obtained by the presented method.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Overall, our data showed that this method is accurate, sensitive, and specific for nucleic acid identification, showing the potential applications for qualitative analysis of respiratory viruses and deafness gene mutation screening.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.9981","type":"journal-article","created":{"date-parts":[[2024,12,27]],"date-time":"2024-12-27T00:34:46Z","timestamp":1735259686000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Rapid Analysis of Nucleic Acids by Matrix\u2010Assisted Laser Desorption Ionization Time\u2010Of\u2010Flight Mass Spectrometry With a Data Algorithm"],"prefix":"10.1002","volume":"39","author":[{"given":"Zhen","family":"Xu","sequence":"first","affiliation":[{"name":"School of Biomedical Engineering (Suzhou), Division of Life Sciences and Medicine University of Science and Technology of China  Suzhou China"},{"name":"Suzhou Institute of Biomedical Engineering and Technology Chinese Academy of Science  Suzhou China"},{"name":"Tianjin Guoke Medical Engineering and Technology Development Co., Ltd.  Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Baolin","family":"Xiong","sequence":"additional","affiliation":[{"name":"School of Biomedical Engineering (Suzhou), Division of Life Sciences and Medicine University of Science and Technology of China  Suzhou China"},{"name":"Suzhou Institute of Biomedical Engineering and Technology Chinese Academy of Science  Suzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenbo","family":"Cheng","sequence":"additional","affiliation":[{"name":"School of Biomedical Engineering (Suzhou), Division of Life Sciences and Medicine University of Science and Technology of China  Suzhou China"},{"name":"Suzhou Institute of Biomedical Engineering and Technology Chinese Academy of Science  Suzhou China"},{"name":"Tianjin Guoke Medical Engineering and Technology Development Co., Ltd.  Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8294-1538","authenticated-orcid":false,"given":"Bin","family":"Hu","sequence":"additional","affiliation":[{"name":"College of Environment and Climate, Institute of Mass Spectrometry and Atmospheric Environment, Guangdong Provincial Engineering Research Center for On\u2010Line Source Apportionment System of Air Pollution Jinan University  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuguo","family":"Tang","sequence":"additional","affiliation":[{"name":"School of Biomedical Engineering (Suzhou), Division of Life Sciences and Medicine University of Science and Technology of China  Suzhou China"},{"name":"Suzhou Institute of Biomedical Engineering and Technology Chinese Academy of Science  Suzhou 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