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This cardiomyopathy contributes to increased occurrence of adverse cardiac events compared with age\u2010matched population norms. Research on cardiac atrophy has focused on remodelling; however, alterations in metabolic properties may be a primary contributor. The purpose of the study is to determine how cancer\u2010induced cardiac atrophy alters mitochondrial turnover, mitochondrial mRNA translation machinery, and in vitro oxidative characteristics.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Lewis lung carcinoma (LLC) tumours were implanted in C57BL6\/J mice and grown for 28\u00a0days to induce cardiac atrophy. Endogenous metabolic species and markers of mitochondrial function were assessed. H9c2 cardiomyocytes were cultured in LLC\u2010conditioned media with (out) the antioxidant MitoTempo. Cells were analysed for reactive oxygen species (ROS), oxidative capacity, and hypoxic resistance.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>LLC heart weights were ~10% lower than controls. LLC hearts demonstrated ~15% lower optical redox ratio [flavin adenine dinucleotide (FAD)\/FAD\u00a0+\u00a0nicotinamide adenine dinucleotide hydrogen (NADH)] compared with phosphate\u2010buffered saline controls. When compared with phosphate\u2010buffered saline, LLC hearts showed ~50% greater Cytochrome\u2010C oxidase subunit 4 (COX\u2010IV) and Voltage\u2010dependent anion channel (VDAC), attributed to ~50% lower mitophagy markers. mt\u2010mRNA translation machinery was elevated similarly to markers of mitochondrial content. Mitochondrial DNA\u2010encoded Cytb was ~30% lower in LLC hearts. ROS scavengers GPx\u20103 and GPx\u20107 were ~50% lower in LLC hearts. Treatment of cardiomyocytes with LLC\u2010conditioned media resulted in higher ROS (25%), lower oxygen consumption rates (10% at basal and 75% at maximal), and greater susceptibility to hypoxia (~25%), which was reversed by MitoTempo.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These results substantiate metabolic cardiotoxic effects attributable to tumour\u2010associated factors and provide insight into interactions between mitochondrial mRNA translation, ROS mitigation, oxidative capacity, and hypoxia resistance.<\/jats:p><\/jats:sec>","DOI":"10.1002\/rco2.18","type":"journal-article","created":{"date-parts":[[2020,8,7]],"date-time":"2020-08-07T19:59:05Z","timestamp":1596830345000},"page":"3-15","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Cancer\u2010induced cardiac atrophy adversely affects myocardial redox state and mitochondrial oxidative 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