{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T04:36:05Z","timestamp":1793162165697,"version":"4.5.0"},"reference-count":30,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T00:00:00Z","timestamp":1792108800000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Robotics Computer Surgery"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Triphenyl phosphate (TPP), a ubiquitous organophosphate flame retardant and plasticiser, has emerged as a pervasive environmental contaminant with suspected endocrine\u2010disrupting properties.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>In this study, we employed an integrated approach combining epigenetic CRISPR\u2010Cas9 knockout screening, spatial transcriptomics, and metabolic flux analysis to explore the response of glioma cells to in vitro exposure to a nominal TPP concentration of 11\u00a0\u03bcM. Targeted cellular, biochemical, promoter\u2010occupancy and signalling assays were performed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>TPP exposure was associated with changes in several cellular readouts in glioma cells, including a higher CCK\u20108 signal, differences in MMP secretion, Rac1 activity and matrix adhesion and differences in glucose, lactate and mitochondrial\u2010respiration readouts. TPP exposure was also associated with increased BRG1 occupancy at the assayed epidermal growth factor receptor (EGFR) promoter region, together with higher EGFR\u2010 and epiregulin (EREG)\u2010related readouts. This pattern is consistent with a candidate EREG\u2013EGFR\/MAPK\u2013ERK pathway association but does not prove a self\u2010sustaining loop or direct chemical recruitment. Spatial transcriptomic analysis described regional heterogeneity in mitochondrial\u2010gene expression within public glioblastoma tissue.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Our findings support a candidate BRG1\u2010associated cellular framework in which nominal TPP exposure is associated with BRG1\u2010related chromatin and EGFR\/metabolic readouts in glioma cells.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcs.70242","type":"journal-article","created":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T14:20:52Z","timestamp":1792160452000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Triphenyl Phosphate Exposure Is Associated With BRG1\u2010Related Cellular and Metabolic Readouts and EREG\u2013EGFR\/ERK Signalling in Glioma Cells"],"prefix":"10.1002","volume":"22","author":[{"given":"Heng","family":"Zhao","sequence":"first","affiliation":[{"name":"Department of Pediatric Neurosurgery Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiwei","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Encephalopathy Taizhou Hospital of Traditional Chinese Medicine  Taizhou Jiangsu Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Pediatric Neurosurgery Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-0913-2671","authenticated-orcid":false,"given":"Fangqing","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Pediatric Neurosurgery Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine  Shanghai 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