{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T10:45:26Z","timestamp":1777632326667,"version":"3.51.4"},"reference-count":34,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T00:00:00Z","timestamp":1770163200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T00:00:00Z","timestamp":1770163200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Respirology"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background and Objective<\/jats:title>\n                    <jats:p>The progression of pulmonary arterial hypertension (PAH) is associated with pulmonary artery (PA) remodelling by M2 macrophages through excessive tissue repair, although some M2 macrophages have a vascular protective role. A quality and quantity culture method (MNC\u2010QQc) increases the number of M2 macrophages in mononuclear cells (MNCs) from peripheral blood. Several studies demonstrate that MNC\u2010QQc has vascular protective effects in ischemic lesions. This study aimed to investigate whether MNC\u2010QQc transplantation ameliorates PAH development in a rat model.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>MNC\u2010QQc extracted from GFP positive donor rats were administered into recipient rats with PAH via the cervical vein. Pulmonary haemodynamics and PA remodelling were assessed. Immunohistochemistry and flow cytometry analysis were performed to identify the donor\u2010derived MNC\u2010QQc cell types in the lungs. The effects of MNC\u2010QQc on the functions of vascular constituent cells were evaluated, and gene expression related to the pathological abnormalities in PA was assessed using microarray analysis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>MNC\u2010QQc transplantation reversed the PAH\u2010induced pulmonary haemodynamics and PA remodelling. GFP\u2010positive MNC\u2010QQc cells, predominantly CD14\u2010positive M2 macrophages, were found around the recipient's PAs. The therapeutic effect was reduced in MNC\u2010QQc without M2 cells. MNC\u2010QQc also enhanced angiogenesis in pulmonary endothelial cells. Microarray analysis of the lung tissue showed that MNC\u2010QQc decreased the expression of Tachykinin Receptor 1, associated with PAH progression, with this reduction linked to CD14\u2010 and CD163\u2010positive M2 macrophages.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      These findings suggest that MNC\u2010QQc, particularly through CD14\u2010 and CD163\u2010positive M2 macrophages, could be a novel treatment for PAH.\n                      <jats:boxed-text content-type=\"graphic\" position=\"anchor\">\n                        <jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/resp70206-gra-0001-m.png\">\n                          <jats:alt-text>image<\/jats:alt-text>\n                        <\/jats:graphic>\n                      <\/jats:boxed-text>\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/resp.70206","type":"journal-article","created":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T06:55:30Z","timestamp":1770274530000},"page":"530-540","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Transplantation of Ex\u00a0Vivo Amplified Mononuclear Cells From Peripheral Blood Improves Rat Pulmonary Hypertension via\n                    <scp>CD14<\/scp>\n                    \u2010 and\n                    <scp>CD163<\/scp>\n                    \u2010Positive\n                    <scp>M2<\/scp>\n                    Macrophages"],"prefix":"10.1002","volume":"31","author":[{"given":"Yoshifumi","family":"Suzuki","sequence":"first","affiliation":[{"name":"Department of Respiratory Medicine Juntendo University Faculty of Medicine and Graduate School of Medicine  Tokyo 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