{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T12:25:39Z","timestamp":1788956739936,"version":"build-2803163510"},"reference-count":29,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T00:00:00Z","timestamp":1774569600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T00:00:00Z","timestamp":1774569600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Respirology"],"published-print":{"date-parts":[[2026,7]]},"abstract":"ABSTRACT<\/jats:title>\n \n Background and Objective<\/jats:title>\n The clinical utility of rapid multiplex PCR panels in patients with exacerbation of COPD (ECOPD) is undefined. We aimed to assess the clinical relevance of the BioFire FilmArray pneumonia plus panel (FAPP) testing in ECOPD in a real\u2010world hospital setting.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n A prospective observational study was conducted on 74 hospitalized ECOPD patients. Spontaneous sputum samples were analysed using FAPP alongside standard\u2010of\u2010care (SOC) tests. Microbial profiles were assessed, and patients were phenotyped based on FAPP findings.<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n FAPP significantly increased the proportion of patients with identifiable pathogens from 36% by SOC tests to 78% (increase of 42%,\n p<\/jats:italic>\n \u2009<\u20090.0001), primarily contributed by increased bacterial detection (increase of 47%,\n p<\/jats:italic>\n \u2009<\u20090.0001), with a shorter turnaround time (median 6\u2009h vs. 75\u2009h,\n p<\/jats:italic>\n \u2009<\u20090.0001).\n \n Staphylococcus aureus<\/jats:italic>\n <\/jats:styled-content>\n was the most prevalent bacteria, while\n \n Haemophilus influenzae<\/jats:italic>\n <\/jats:styled-content>\n predominated at high abundance (10\n 6<\/jats:sup>\n \u2013\u2265\u200910\n 7<\/jats:sup>\n copies\/ml). Respiratory viruses were detected in 27%, most frequently Influenza A. All in\u2010panel cultured bacteria were concordantly detected by FAPP at high abundance. High\u2010abundance bacterial detection was associated with significantly higher host immune response markers. The bacterial\u2010predominant phenotype was associated with higher exacerbation severity, while the purely eosinophilic phenotype had a lower 180\u2010day treatment failure rate compared to the unclassified phenotype.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n \n FAPP significantly enhances microbial detection in patients with ECOPD. Appropriate utilization of FAPP might guide antibiotic decision\u2010making and phenotyping in ECOPD. The potential of sputum multiplex PCR\u2010based strategy to guide antibiotic decisions in ECOPD merits further research.\n \n \n image<\/jats:alt-text>\n <\/jats:graphic>\n <\/jats:boxed-text>\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/resp.70242","type":"journal-article","created":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T08:35:23Z","timestamp":1774600523000},"page":"702-710","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Real\u2010World Evaluation of a Rapid Multiplex\n PCR<\/scp>\n Panel in Adults Hospitalized With\n COPD<\/scp>\n Exacerbations"],"prefix":"10.1002","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3336-2414","authenticated-orcid":false,"given":"Charles","family":"Wong","sequence":"first","affiliation":[{"name":"Department of Medicine Pamela Youde Nethersole Eastern Hospital Hong Kong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiu Wing","family":"Lam","sequence":"additional","affiliation":[{"name":"Department of Clinical Pathology Pamela Youde Nethersole Eastern Hospital Hong Kong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vinson Nelson","family":"Yew","sequence":"additional","affiliation":[{"name":"Department of Medicine Pamela Youde Nethersole Eastern Hospital Hong Kong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey Chi Chung","family":"Wong","sequence":"additional","affiliation":[{"name":"Department of Medicine Pamela Youde Nethersole Eastern Hospital Hong Kong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3537-9535","authenticated-orcid":false,"given":"Hei Shun","family":"Cheng","sequence":"additional","affiliation":[{"name":"Department of Medicine Pamela Youde Nethersole Eastern Hospital Hong Kong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pui Hing","family":"Chiu","sequence":"additional","affiliation":[{"name":"Department of Medicine Pamela Youde Nethersole Eastern Hospital Hong Kong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun Wai","family":"Tong","sequence":"additional","affiliation":[{"name":"Department of Medicine Pamela Youde Nethersole Eastern Hospital Hong Kong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Flora Pui Ling","family":"Miu","sequence":"additional","affiliation":[{"name":"Department of Medicine Pamela Youde Nethersole Eastern Hospital Hong Kong China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,3,27]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamanetworkopen.2023.46598"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.157.5.9709032"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201605-1014OC"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.202209-1748SO"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rmed.2004.09.011"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0954-6111(03)00026-X"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-106-2-196"},{"key":"e_1_2_11_9_1","unstructured":"Global Initiative for Chronic Obstructive Lung Disease \u201cGlobal Strategy for the Diagnosis Management and Prevention of Chronic Obstructive Pulmonary Disease. 2023 Report\u201d https:\/\/goldcopd.org\/2023\u2010gold\u2010report\u20102\/."},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(24)01867-1"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmjph-2024-002411"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.accpm.2023.101300"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2020.11.014"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00128-20"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13054-020-03114-y"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmii.2019.10.009"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1177\/17534666251341751"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2020.03.024"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12941-022-00512-8"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiad221"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.6.4.396-399.1977"},{"key":"e_1_2_11_22_1","first-page":"24","volume-title":"Medical Microbiology: Quality, Cost and Clinical Relevance","author":"Bartlett R. 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