{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T09:36:08Z","timestamp":1771061768360,"version":"3.50.1"},"reference-count":42,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,10,11]],"date-time":"2018-10-11T00:00:00Z","timestamp":1539216000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Medicine &amp; Biology"],"published-print":{"date-parts":[[2019,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p><jats:italic>Microtus<\/jats:italic> genus is one of the experimental animals showing unique characteristics, and some species have been used as various research models. In order to advance the utilization of <jats:italic>Microtus<\/jats:italic> genus, the development of assisted reproductive technologies (ARTs) is a key point. This review introduces recent progress in the development of ARTs for <jats:italic>Microtus<\/jats:italic> genus, especially <jats:italic>Microtus montebelli<\/jats:italic> (Japanese field vole).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Based on previous and our publications, current status of the development of ARTs was summarized.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In <jats:italic>M<\/jats:italic>.<jats:italic> montebelli<\/jats:italic>, ARTs, such as superovulation, in vitro fertilization, intracytoplasmic sperm injection, embryo transfer, sperm cryopreservation, and nonsurgical artificial insemination, have considerably been established by using the procedures which were originally devised for mice and partly modified. However, when the methods for <jats:italic>M<\/jats:italic>.<jats:italic> montebelli<\/jats:italic> were applied to <jats:italic>Microtus arvalis<\/jats:italic> and <jats:italic>Microtus rossiaemeridionalis<\/jats:italic>, all protocols of ARTs except for sperm cryopreservation were technologically invalid.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Assisted reproductive technologies (ARTs) are considerably established in <jats:italic>M<\/jats:italic>.<jats:italic> montebelli<\/jats:italic>, and this fact allows this species to be potentially useful as a model animal. However, since ART protocols of <jats:italic>M<\/jats:italic>.<jats:italic> montebelli<\/jats:italic> are mostly invalid for other species of the <jats:italic>Microtus<\/jats:italic> genus, it is necessary to improve them specifically for each of other species.<\/jats:p><\/jats:sec>","DOI":"10.1002\/rmb2.12244","type":"journal-article","created":{"date-parts":[[2018,10,12]],"date-time":"2018-10-12T05:54:23Z","timestamp":1539323663000},"page":"121-127","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Assisted reproductive technologies in <i>Microtus<\/i> genus"],"prefix":"10.1002","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2141-4662","authenticated-orcid":false,"given":"Konosuke","family":"Okada","sequence":"first","affiliation":[{"name":"Graduate School of Veterinary Medicine and Life Science, Graduate School Nippon Veterinary and Life Science University Musashino, Tokyo 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