{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T08:07:42Z","timestamp":1771920462437,"version":"3.50.1"},"reference-count":44,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,9,21]],"date-time":"2019-09-21T00:00:00Z","timestamp":1569024000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"JSPS KAKENHI","award":["22580316"],"award-info":[{"award-number":["22580316"]}]},{"name":"JSPS KAKENHI","award":["25450390"],"award-info":[{"award-number":["25450390"]}]},{"name":"JSPS KAKENHI","award":["18K05936"],"award-info":[{"award-number":["18K05936"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Medicine &amp; Biology"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Purpose<\/jats:title><jats:p>This study aimed to examine whether the Tinagl1 might be associated with ovulation in aged females and reproductive age\u2010associated fibrosis in the stroma of the ovary.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>To address the ovulatory ability and quality of ovulated oocytes, we induced ovulation by treatment with equine chorionic gonadotropin (eCG) and human chorionic gonadotropin (hCG) followed by in vitro fertilization. We also performed Picrosirius Red (PSR) staining to evaluate ovarian collagen deposition.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>As compared to ovulation in 8\u2010 to 9\u2010month\u2010old <jats:italic>Tinagl1<jats:sup>flox\/flox<\/jats:sup><\/jats:italic> mice, the number of ovulated oocytes from <jats:italic>Tinagl1<jats:sup>flox\/flox<\/jats:sup><\/jats:italic> mice decreased in an age\u2010dependent manner in mice more than 10\u201011\u00a0months old, whereas the ovulated oocyte numbers in <jats:italic>Tinagl1<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> mice decreased significantly at 14\u201015\u00a0months. In vitro fertilization followed by embryo culture demonstrated the normal developmental potential of <jats:italic>Tinagl1<\/jats:italic>\u2010null embryos during the preimplantation period. PSR staining indicated that collagen was found throughout the ovarian stroma in an age\u2010dependent manner in <jats:italic>Tinagl1<jats:sup>flox\/flox<\/jats:sup><\/jats:italic> females, whereas those distributions were delayed to 14\u201015\u00a0months in <jats:italic>Tinagl1<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> females. This timing was consistent with the delayed timing of age\u2010related decline of ovulation in <jats:italic>Tinagl1<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> females.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The alleviation of age\u2010associated depression of ovulation was caused by delayed ovarian collagen deposition in <jats:italic>Tinagl1<\/jats:italic>\u2010null female mice.<\/jats:p><\/jats:sec>","DOI":"10.1002\/rmb2.12301","type":"journal-article","created":{"date-parts":[[2019,9,21]],"date-time":"2019-09-21T07:42:41Z","timestamp":1569051761000},"page":"50-57","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Tubulointerstitial nephritis antigen\u2010like 1 deficiency alleviates age\u2010dependent depressed ovulation associated with ovarian collagen deposition in mice"],"prefix":"10.1002","volume":"19","author":[{"given":"Masato","family":"Akaiwa","sequence":"first","affiliation":[{"name":"Laboratory of Animal Breeding and Reproduction Division of Animal Science School of Agriculture Utsunomiya University Utsunomiya Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emiko","family":"Fukui","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Breeding and Reproduction Division of Animal Science School of Agriculture Utsunomiya University Utsunomiya Japan"},{"name":"Center for Bioscience Research and Education Utsunomiya University Utsunomiya Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0104-6866","authenticated-orcid":false,"given":"Hiromichi","family":"Matsumoto","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Breeding and Reproduction Division of Animal Science School of Agriculture Utsunomiya University Utsunomiya 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