{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T12:18:48Z","timestamp":1777033128633,"version":"3.51.4"},"reference-count":23,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2020,7,20]],"date-time":"2020-07-20T00:00:00Z","timestamp":1595203200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Saitama Medical University Hospital for young scientists","award":["29E108"],"award-info":[{"award-number":["29E108"]}]},{"name":"JSPS KAKENHI Grant","award":["16K20204"],"award-info":[{"award-number":["16K20204"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Medicine &amp; Biology"],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Purpose<\/jats:title><jats:p>Subendometrial myometrium exerts wave\u2010like activity throughout the menstrual cycle, and uterine peristalsis is markedly reduced during the implantation phase. We hypothesized that abnormal uterine peristalsis has an adverse effect on the endometrial decidualization process. We conducted an in vitro culture experiment to investigate the effect of cyclic stretch on the morphological and biological endometrial decidual process.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Primary human endometrial stromal cells (HESCs) were isolated from hysterectomy specimens and incubated with or without 8\u2010bromo\u2010cyclic adenosine monophosphate (8\u2010br\u2010cAMP) and medroxyprogesterone acetate (MPA) for 3\u00a0days. After decidualization, cultures were continued for 24\u00a0hours with or without cyclic stretch using a computer\u2010operated cell tension system.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Cyclic stretch significantly repressed expression of decidual markers including insulin\u2010like growth factor\u2010binding protein 1 (<jats:italic>IGFBP1<\/jats:italic>), prolactin (<jats:italic>PRL<\/jats:italic>), forkhead box O1 (<jats:italic>FOXO1<\/jats:italic>), and <jats:italic>WNT4<\/jats:italic> on decidualized HESCs. In addition, cyclic stretch of decidualized HESCs affected the decidual morphological phenotype to an elongated shape. The alternation of F\u2010actin localization in decidualized HESCs was not observed in response to cyclic stretch.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These data suggest that cyclic stretch inhibits the morphological and biological decidual process of HESCs. Our findings imply that uterine abnormal contractions during the implantation period impair endometrial decidualization and contribute to infertility.<\/jats:p><\/jats:sec>","DOI":"10.1002\/rmb2.12341","type":"journal-article","created":{"date-parts":[[2020,7,20]],"date-time":"2020-07-20T13:14:09Z","timestamp":1595250849000},"page":"378-384","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["High stretch cycling inhibits the morphological and biological decidual process in human endometrial stromal cells"],"prefix":"10.1002","volume":"19","author":[{"given":"Ryohei","family":"Saito","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology Saitama Medical University Iruma\u2010gun Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4853-9297","authenticated-orcid":false,"given":"Takeshi","family":"Kajihara","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Saitama Medical University Iruma\u2010gun Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masashi","family":"Takamura","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Saitama Medical University Iruma\u2010gun Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hideno","family":"Tochigi","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Saitama Medical University Iruma\u2010gun Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tsuyoshi","family":"Sato","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery Saitama Medical University Iruma\u2010gun Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Osamu","family":"Ishihara","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Saitama Medical University Iruma\u2010gun Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,7,20]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00795-013-0018-z"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1677\/joe.0.1780357"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2007-991042"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1210\/me.2007-0058"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1507\/endocrj.K08E-067"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.140.10.7070"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-0760(97)80004-0"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9378(81)90237-4"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejogrb.2006.07.040"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/deq222"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmig.2011.09.010"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00334.2005"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1210\/me.2005-0275"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M201018200"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep40001"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bb.17.060188.002145"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/rmb2.12028"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/btpr.1883"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21031082"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.140.12.7184"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2006-1673"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2005.08.046"}],"container-title":["Reproductive Medicine and Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Frmb2.12341","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/rmb2.12341","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/rmb2.12341","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/rmb2.12341","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,4]],"date-time":"2023-09-04T15:50:05Z","timestamp":1693842605000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/rmb2.12341"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,7,20]]},"references-count":23,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2020,10]]}},"alternative-id":["10.1002\/rmb2.12341"],"URL":"https:\/\/doi.org\/10.1002\/rmb2.12341","archive":["Portico"],"relation":{},"ISSN":["1445-5781","1447-0578"],"issn-type":[{"value":"1445-5781","type":"print"},{"value":"1447-0578","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,7,20]]},"assertion":[{"value":"2020-05-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-07-02","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-07-20","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}