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The present study investigated the effects of 17\u03b2\u2010estradiol on in vitro maturation of buffalo and goat oocytes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Cumulus oocyte complexes (COCs) were aspirated from large antral follicles of slaughtered buffalo and goat ovaries. COCs were cultured in TCM\u2010199 medium supplemented with 0, 0.5, 1, and 1.5\u00a0\u00b5g\/mL of 17\u03b2\u2010estradiol for in vitro maturation. Then, oocytes were used for the examination of state of nuclear maturation and cumulus expansion.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In both species, oocytes treated with 17\u03b2\u2010estradiol showed higher cumulus expansion rate than control (0\u00a0\u00b5g\/mL treated). In buffalo, the percentage of oocytes matured to the metaphase II (MII) stage increased in the concentration\u2010dependent manner of 17\u03b2\u2010estradiol. Similarly, estradiol positively influenced nuclear maturation of goat oocytes in vitro.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Estradiol has promoting effects on normalprogress of in vitro oocyte meiosis in buffalos and goats.<\/jats:p><\/jats:sec>","DOI":"10.1002\/rmb2.12350","type":"journal-article","created":{"date-parts":[[2020,9,26]],"date-time":"2020-09-26T06:22:03Z","timestamp":1601101323000},"page":"62-70","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Effects of estradiol on in vitro maturation of buffalo and goat oocytes"],"prefix":"10.1002","volume":"20","author":[{"given":"Hurum","family":"Maksura","sequence":"first","affiliation":[{"name":"Department of Animal Science Bangladesh Agricultural University  Mymensingh 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