{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T16:11:44Z","timestamp":1778602304446,"version":"3.51.4"},"reference-count":14,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,10,8]],"date-time":"2020-10-08T00:00:00Z","timestamp":1602115200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["19H00459"],"award-info":[{"award-number":["19H00459"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Medicine &amp; Biology"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Purpose<\/jats:title><jats:p>Chromosomal abnormalities are a major cause of spontaneous abortion, and conventional G\u2010banded karyotyping (G\u2010banding) is mainly utilized for chromosomal analysis. Recently, next\u2010generation sequencing (NGS) has been introduced for chromosomal analysis. Here, we aimed to investigate the applicability and utility of NGS\u2010based chromosomal analysis of products of conception (POC) on chorionic villus samples from spontaneous abortion.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The results of chromosomal analysis of 7 chorionic villus samples from spontaneous abortion were compared between conventional G\u2010banding and NGS\u2010based chromosomal copy number analysis. Age dependency and frequency of each chromosomal aneuploidy were evaluated for 279 cases analyzed by NGS.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Excluding two cases (culture failure and maternal cell contamination), the results were consistent between G\u2010banding and NGS. For cases analyzed by NGS, the rate of chromosomal abnormality increased in a maternal age\u2010dependent manner. The frequency of each chromosomal aneuploidy detected by NGS was almost the same as that previously reported. Finally, NGS analysis was possible for difficult cases by G\u2010banding analysis, such as culture failure, maternal cell contamination, long\u2010term storage cases, and low cell number.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Chromosome analysis using NGS not only obtains comparable results to conventional G\u2010banding, but also can analyze POC more accurately and efficiently.<\/jats:p><\/jats:sec>","DOI":"10.1002\/rmb2.12351","type":"journal-article","created":{"date-parts":[[2020,10,9]],"date-time":"2020-10-09T05:36:57Z","timestamp":1602221817000},"page":"71-75","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Chromosomal copy number analysis of products of conception by conventional karyotyping and next\u2010generation sequencing"],"prefix":"10.1002","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2423-8614","authenticated-orcid":false,"given":"Yuki","family":"Tamura","sequence":"first","affiliation":[{"name":"Nippon Reprogenetics Inc.  Maebashi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mitsuo","family":"Santo","sequence":"additional","affiliation":[{"name":"Nippon Reprogenetics Inc.  Maebashi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasuhisa","family":"Araki","sequence":"additional","affiliation":[{"name":"Nippon Reprogenetics Inc.  Maebashi Japan"},{"name":"Graduate School of Health Science Gunma Paz University  Takasaki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hidehiko","family":"Matsubayashi","sequence":"additional","affiliation":[{"name":"Reproduction Clinic Osaka  Osaka Japan"},{"name":"Reproduction Clinic Tokyo  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yukiko","family":"Takaya","sequence":"additional","affiliation":[{"name":"Reproduction Clinic Osaka  Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kotaro","family":"Kitaya","sequence":"additional","affiliation":[{"name":"Reproduction Clinic Osaka  Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masakazu","family":"Doshida","sequence":"additional","affiliation":[{"name":"Reproduction Clinic Tokyo  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1977-923X","authenticated-orcid":false,"given":"Kohei","family":"Yamaguchi","sequence":"additional","affiliation":[{"name":"Reproduction Clinic Tokyo  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shimpei","family":"Mizuta","sequence":"additional","affiliation":[{"name":"Reproduction Clinic Osaka  Osaka Japan"},{"name":"Reproduction Clinic Tokyo  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chie","family":"Takahashi","sequence":"additional","affiliation":[{"name":"Reproduction Clinic Tokyo  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Namhyo","family":"Kim","sequence":"additional","affiliation":[{"name":"Reproduction Clinic Osaka  Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Koichiro","family":"Okuno","sequence":"additional","affiliation":[{"name":"Reproduction Clinic Osaka  Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takumi","family":"Takeuchi","sequence":"additional","affiliation":[{"name":"Reproduction Clinic Tokyo  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomomoto","family":"Ishikawa","sequence":"additional","affiliation":[{"name":"Reproduction Clinic Osaka  Osaka Japan"},{"name":"Reproduction Clinic Tokyo  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,10,8]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/1471-0528.12382"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1159\/000477707"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2017.01.022"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1590\/S1415-47572008000300004"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2013.09.031"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dey021"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13039-015-0210-z"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.5582\/bst.2017.01296"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1097\/MD.0000000000018041"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2019.07.1371"},{"key":"e_1_2_9_12_1","unstructured":"PGDIS position statement on chromosome mosaicism and preimplantation aneuploidy testing at the blastocyst stage. PGDIS Newsletter July 19 2016.https:\/\/www.pgdis.org\/docs\/newsletter_071816.html. Accessed March 16 2020."},{"key":"e_1_2_9_13_1","first-page":"e1","article-title":"PGDIS position statement on the transfer of mosaic embryos 2019","volume":"39","author":"Cram DS","year":"2019","journal-title":"RBM Online"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2014.07.098"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2011.07.135"}],"container-title":["Reproductive Medicine and Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/rmb2.12351","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/rmb2.12351","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/rmb2.12351","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,3]],"date-time":"2023-09-03T15:41:40Z","timestamp":1693755700000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/rmb2.12351"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,10,8]]},"references-count":14,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2021,1]]}},"alternative-id":["10.1002\/rmb2.12351"],"URL":"https:\/\/doi.org\/10.1002\/rmb2.12351","archive":["Portico"],"relation":{},"ISSN":["1445-5781","1447-0578"],"issn-type":[{"value":"1445-5781","type":"print"},{"value":"1447-0578","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,10,8]]},"assertion":[{"value":"2020-05-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-09-14","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-10-08","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}