{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,16]],"date-time":"2025-12-16T11:58:37Z","timestamp":1765886317428,"version":"3.41.2"},"reference-count":36,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,10,27]],"date-time":"2021-10-27T00:00:00Z","timestamp":1635292800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Medicine &amp; Biology"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Purpose<\/jats:title><jats:p>To determine the utility of short gamete coincubation in <jats:italic>in vitro<\/jats:italic> fertilization (IVF\u2010S) combined with early rescue intracytoplasmic sperm injection (R\u2010ICSI) and split IVF\u2010ICSI in preventing low fertilization based on a retrospective cohort study.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Couples with a high risk of low IVF fertilization during the first ART cycle underwent IVF\u2010S with R\u2010ICSI or split IVF\u2010ICSI. Fertilization rate, embryo quality, and clinical outcomes were measured.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>After propensity score matching, we included 188 couples in the IVF\u2010S with R\u2010ICSI group as Group 1 and 720 in the split IVF\u2010ICSI group as Group 2. Normal fertilization rates were similar; however, Group 1\u00a0had a higher multiple pronuclei rate (10.42% vs. 4.50%, <jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.001) but a higher embryo utilization rate (59.84% vs. 53.60%, <jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.001). The groups were similar in the rates of high\u2010quality embryos, embryo implantation, clinical pregnancy, and live birth. Low IVF fertilization rate was 4.79% and 9.03% in Group 1 and Group 2, respectively, with similar fertilization rate and embryo development.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>IVF\u2010S with early R\u2010ICSI and split IVF\u2010ICSI were effective strategies in preventing low fertilization rate. IVF\u2010S with early R\u2010ICSI could become the preferred approach because of its advantages\u2014higher embryo utilization rate, fewer ICSI procedures, similar clinical pregnancy rate, and live birth rate.<\/jats:p><\/jats:sec>","DOI":"10.1002\/rmb2.12420","type":"journal-article","created":{"date-parts":[[2021,10,27]],"date-time":"2021-10-27T13:48:47Z","timestamp":1635342527000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Effect of early rescue ICSI and split IVF\u2010ICSI in preventing low fertilization rate during the first ART cycle: A real\u2010world retrospective cohort study"],"prefix":"10.1002","volume":"21","author":[{"given":"Linlin","family":"Jiang","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology Sun Yat\u2010Sen Memorial Hospital Sun Yat\u2010Sen University Guangzhou 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