{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,12]],"date-time":"2026-01-12T21:06:54Z","timestamp":1768252014697,"version":"3.49.0"},"reference-count":30,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,12,13]],"date-time":"2021-12-13T00:00:00Z","timestamp":1639353600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Medicine &amp; Biology"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Purpose<\/jats:title><jats:p>Early rescue intracytoplasmic sperm injection (ICSI) is often performed in cases in which not even a single oocyte has extruded a second polar body 6\u00a0h after insemination. We evaluated the usefulness of expanding the indications of early rescue ICSI to cases in which &lt;80% of oocytes have extruded second polar bodies 6\u00a0h after insemination.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Early rescue ICSI was performed on oocytes that were denuded 2.5\u00a0h post\u2010insemination and whose extrusion of the second polar bodies had been examined 6\u00a0h post\u2010insemination with a PolScope.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In vitro fertilization was performed on 24\u00a0496 oocytes of 4944 cycles, and 1438 cycles had &lt;80% rate of the second polar body extrusion. Rescue ICSI was performed on 3933 oocytes. Three pronuclei (3PN) incidence of rescue ICSI was 3.0% in oocytes with \u226550% rate of the second polar body extrusion. With respect to the second polar body extrusion rate, no differences were observed in normal fertilization, blastocyst development, implantation, miscarriage, or live birth rates for rescue ICSI.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>By expanding the indications of early rescue ICSI using the PolScope to cases in which &lt;80% of oocytes have extruded the second polar bodies, many fertilized oocytes can be obtained without considerably increasing the 3PN rate.<\/jats:p><\/jats:sec>","DOI":"10.1002\/rmb2.12432","type":"journal-article","created":{"date-parts":[[2021,12,13]],"date-time":"2021-12-13T12:49:59Z","timestamp":1639399799000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Usefulness of expanding the indications of early rescue intracytoplasmic sperm 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