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In mice, after blastocysts enter the uterine cavities on day 4 of pregnancy (day 1\u00a0=\u00a0vaginal plug), blastocyst attachment is completed within 24\u00a0h, accompanied by dynamic interactions between the uterine luminal epithelium and the blastocysts. Any failures in this process compromise subsequent pregnancy outcomes. Here, we performed comprehensive analyses of gene expression at the luminal epithelium in the peri\u2010implantation period.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>RNA\u2010seq combined with laser microdissection (LMD) was used to reveal unique gene expression kinetics in the epithelium.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We found that the prereceptive epithelium on day 3 specifically expresses cell cycle\u2010related genes. In addition, days 3 and 4 epithelia express glutathione pathway\u2010related genes, which are protective against oxidative stresses. In contrast, day 5 epithelium expresses genes involved in glycolysis and the regulation of cell proliferation. The genes highly expressed on days 3 and 4 compared to day 5 are related to progesterone receptor signaling, and the genes highly expressed on day 5 compared to days 3 and 4 are associated with the ones regulated by H3K27me3.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These results suggest that specific gene expression patterns govern uterine functions during early pregnancy, contributing to implantation success.<\/jats:p><\/jats:sec>","DOI":"10.1002\/rmb2.12435","type":"journal-article","created":{"date-parts":[[2022,1,5]],"date-time":"2022-01-05T11:58:53Z","timestamp":1641383933000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["A gene network of uterine luminal epithelium organizes mouse blastocyst implantation"],"prefix":"10.1002","volume":"21","author":[{"given":"Shizu","family":"Aikawa","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology 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