{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T00:19:39Z","timestamp":1784765979986,"version":"3.55.0"},"reference-count":40,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,1,29]],"date-time":"2022-01-29T00:00:00Z","timestamp":1643414400000},"content-version":"vor","delay-in-days":28,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Medicine &amp; Biology"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Purpose<\/jats:title><jats:p>To investigate the relationship between the microbiome of the female genital tract and endometriosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>This prospective cohort study included 36 women who underwent laparoscopic surgery for ovarian tumor from July 2019 to April 2020. Of them, 18 had endometriosis, and 18 did not have endometriosis. Vaginal secretions, endometrial fluid, peritoneal fluid, and ovarian cystic fluid were collected during surgery. Next\u2010generation sequencing of bacterial 16S rRNA was performed to characterize the microbiome.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Specific microbiomes were not detected in either peritoneal fluid or ovarian cystic fluid regardless of the presence or absence of endometriosis and the type of cyst. When the cutoff value of infectious bacterial abundance in the vagina was set as 64.3%, there were many cases more than a cutoff value in the endometriosis group significantly (<jats:italic>p<\/jats:italic>\u00a0=\u00a00.01). When the cutoff value of infectious bacterial abundance in the endometrium was set as 18.6%, there were many cases more than a cutoff level in the endometriosis cases significantly (<jats:italic>p<\/jats:italic>\u00a0=\u00a00.02).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Peritoneal fluid and ovarian cystic fluid are almost sterile, although dysbiosis may occur in the vaginal and endometrial microbiome in women with endometriosis.<\/jats:p><\/jats:sec>","DOI":"10.1002\/rmb2.12441","type":"journal-article","created":{"date-parts":[[2022,1,29]],"date-time":"2022-01-29T12:04:56Z","timestamp":1643457896000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Microbiome analysis in women with endometriosis: Does a microbiome exist in peritoneal fluid and ovarian cystic fluid?"],"prefix":"10.1002","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9304-6782","authenticated-orcid":false,"given":"Sugiko","family":"Oishi","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology Graduate School of Medical Science University of the Ryukyus  Okinawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keiko","family":"Mekaru","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Graduate School of Medical Science University of the Ryukyus  Okinawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suguru E.","family":"Tanaka","sequence":"additional","affiliation":[{"name":"Varinos, Inc.  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wataru","family":"Arai","sequence":"additional","affiliation":[{"name":"Varinos, Inc.  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyota","family":"Ashikawa","sequence":"additional","affiliation":[{"name":"Varinos, Inc.  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshiyuki","family":"Sakuraba","sequence":"additional","affiliation":[{"name":"Varinos, Inc.  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mikiko","family":"Nishioka","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Mie University  Mie Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rie","family":"Nakamura","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Graduate School of Medical Science University of the Ryukyus  Okinawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8154-061X","authenticated-orcid":false,"given":"Maho","family":"Miyagi","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Graduate School of Medical Science University of the Ryukyus  Okinawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0831-9900","authenticated-orcid":false,"given":"Kozue","family":"Akamine","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Graduate School of Medical Science University of the Ryukyus  Okinawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoichi","family":"Aoki","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Graduate School of Medical Science University of the Ryukyus  Okinawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,1,29]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.2147\/IDR.S227883"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2011.05.087"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/ajo.12189"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2017.05.031"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2017.05.034"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2018.04.041"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2007.12"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-017-00901-0"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/rmb2.12105"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro3451"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00795-021-00298-2"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/16.Supplement_4.S273"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.3.624-627.1998"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/14.9.2222"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.55.12.961"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1086\/423963"},{"key":"e_1_2_10_18_1","first-page":"28","article-title":"Possible role of enterotoxigenic Bacteroides fragilis in the etiology of infectious vaginitis","volume":"53","author":"Polanco N","year":"2012","journal-title":"Invest Clin"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.2174\/1874285801610010027"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00075-15"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anaerobe.2017.05.005"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00091-16"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpubh.2018.00078"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.2147\/IJWH.S52429"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9378(15)30003-X"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2010.04.053"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejogrb.2016.01.040"},{"key":"e_1_2_10_28_1","first-page":"193","article-title":"Possible role of microbiome in the pathogenesis of endometriosis","volume":"73","author":"D'Alterio MN","year":"2021","journal-title":"Minerva Obstet Gynecol"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2016.09.075"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature09646"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dex372"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dmz044"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2015.08.058"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dmy012"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(01)01816-7"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpobgyn.2018.01.006"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2018.07.004"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-03968-5"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dem399"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00404-020-05679-z"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejogrb.2021.07.014"}],"container-title":["Reproductive Medicine and Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/rmb2.12441","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/rmb2.12441","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/rmb2.12441","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,19]],"date-time":"2023-08-19T22:20:23Z","timestamp":1692483623000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/rmb2.12441"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,1]]},"references-count":40,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2022,1]]}},"alternative-id":["10.1002\/rmb2.12441"],"URL":"https:\/\/doi.org\/10.1002\/rmb2.12441","archive":["Portico"],"relation":{},"ISSN":["1445-5781","1447-0578"],"issn-type":[{"value":"1445-5781","type":"print"},{"value":"1447-0578","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,1]]},"assertion":[{"value":"2021-10-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-01-14","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-01-29","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e12441"}}