{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,27]],"date-time":"2025-08-27T15:32:44Z","timestamp":1756308764411,"version":"3.41.2"},"reference-count":30,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,4,6]],"date-time":"2022-04-06T00:00:00Z","timestamp":1649203200000},"content-version":"vor","delay-in-days":95,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Medicine &amp; Biology"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Purpose<\/jats:title><jats:p>ML\u20102\u20133 is a novel tetracyclic iridoid derived from <jats:italic>Morinda lucida<\/jats:italic> Bentham leaves. This compound has anti\u2010trypanosomal and anti\u2010leishmanial effects. In this study, the authors investigated effects of ML\u20102\u20133 on in vitro fertilization (IVF) rates, motility, and acrosome reaction of the mouse sperm.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>IVF was performed using sperm from BALB\/cByJJcl mice treated with ML\u20102\u20133. Computer\u2010assisted sperm analysis (CASA) was performed on the sperm of C57BL\/6J mice to investigate sperm motility. The effect of ML\u20102\u20133 on the acrosome reaction was examined by observing the fluorescence of sperm labeled with the acrosin\u2010EGFP transgene.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>ML\u20102\u20133 improved IVF in BALB\/cByJJcl mice with low fertilization rates. The optimum concentration of ML\u20102\u20133 in sperm pre\u2010culture medium was 20\u00a0<jats:italic>\u00b5<\/jats:italic>M, and no significant toxicity of ML\u20102\u20133 was observed in developing embryos at this concentration. ML\u20102\u20133 affected sperm motility but not the acrosome reaction. ML\u20102\u20133 increased the IVF rate of mouse sperm that had been refrigerated for 3\u00a0days.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>ML\u20102\u20133 can improve the outcome of IVF and motility without inducing the acrosome reaction in mice. These effects of ML\u20102\u20133 on sperm behaviors are different from those of the similar drugs.<\/jats:p><\/jats:sec>","DOI":"10.1002\/rmb2.12455","type":"journal-article","created":{"date-parts":[[2022,4,7]],"date-time":"2022-04-07T03:49:21Z","timestamp":1649303361000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["In vitro fertilization using sperm activated by ML\u20102\u20133 isolated from <i>Morinda lucida<\/i> Bentham leaves"],"prefix":"10.1002","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4698-651X","authenticated-orcid":false,"given":"Tomoe","family":"Ohta","sequence":"first","affiliation":[{"name":"Department of Pharmacognosy Faculty of Pharmaceutical Sciences Nagasaki International University  Nagasaki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0450-2123","authenticated-orcid":false,"given":"Takuhiro","family":"Uto","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy Faculty of Pharmaceutical Sciences Nagasaki International University  Nagasaki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7190-0258","authenticated-orcid":false,"given":"Yukihiro","family":"Shoyama","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy Faculty of Pharmaceutical Sciences Nagasaki International University  Nagasaki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9058-9570","authenticated-orcid":false,"given":"Maxwell Mamfe","family":"Sakyiamah","sequence":"additional","affiliation":[{"name":"Centre for Plant Medicine Research  Mampong \u2010 Akuapem Ghana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alfred Ampomah","family":"Appiah","sequence":"additional","affiliation":[{"name":"Centre for Plant Medicine Research  Mampong \u2010 Akuapem Ghana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0170-5323","authenticated-orcid":false,"given":"Hiromitsu","family":"Tanaka","sequence":"additional","affiliation":[{"name":"Lab of Molecular Biology Faculty of Pharmaceutical Sciences Nagasaki International University  Nagasaki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,4,6]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/rmb2.12065"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2021.08.014"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/aja.2011.65"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0730441"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(98)00146-0"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939\u20104640.1991.tb00258.x"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.2174\/1874255601406010001"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2015.09.088"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tetlet.2015.11.031"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmcl.2015.05.003"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01916\u201015"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/s41182\u2010016\u20100026\u20105"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2018.10.044"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014\u20105793%2899%2900433\u20100"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod55.3.709"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod61.3.809"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1993.1166"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3027891"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/biolre\/ioy028"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(16)54976-0"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/978\u20103\u2010319\u201030567\u20107_5"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/3.5.403"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.5980\/jpnjurol1989.89.641"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.humrep.a137990"},{"key":"e_1_2_8_26_1","first-page":"192","article-title":"Pentoxifylline induces hyperactivation and acrosome reaction in spermatozoa of golden hamsters: changes in motility kinematics","volume":"2","author":"Jayaprakash D","year":"1997","journal-title":"Hum Reprod"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(16)56108-1"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox10060874"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/rmb2.12251"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox10071096"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-02166-6"}],"container-title":["Reproductive Medicine and Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/rmb2.12455","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/rmb2.12455","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/rmb2.12455","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,19]],"date-time":"2023-08-19T22:16:25Z","timestamp":1692483385000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/rmb2.12455"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,1]]},"references-count":30,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2022,1]]}},"alternative-id":["10.1002\/rmb2.12455"],"URL":"https:\/\/doi.org\/10.1002\/rmb2.12455","archive":["Portico"],"relation":{},"ISSN":["1445-5781","1447-0578"],"issn-type":[{"type":"print","value":"1445-5781"},{"type":"electronic","value":"1447-0578"}],"subject":[],"published":{"date-parts":[[2022,1]]},"assertion":[{"value":"2022-02-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-03-18","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-04-06","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e12455"}}