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Recently, it was reported that <jats:italic>Tulp2<\/jats:italic> knockout (KO) mice exhibited disrupted sperm tail morphology; however, it remains to be determined how TULP2 deletion causes abnormal tail formation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The authors analyzed male fertility, sperm morphology, and motility of two <jats:italic>Tulp2<\/jats:italic> KO mouse lines that were generated using the conventional method that utilizes homologous recombination in embryonic stem (ES) cells as well as the clustered regularly interspaced short palindromic repeats\/CRISPR\u2010associated protein 9 (CRISPR\/Cas9) system. Furthermore, the authors observed the spermatogenesis of <jats:italic>Tulp2<\/jats:italic> KO mice in more detail using scanning and transmission electron microscopy (SEM and TEM).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Both mouse lines of <jats:italic>Tulp2<\/jats:italic> KO exhibited male infertility, abnormal tail morphology, and impaired sperm motility. No overt abnormalities were found in the formation of the mitochondrial sheath in <jats:italic>Tulp2<\/jats:italic> KO mice using the freeze\u2010fracture method with SEM. In contrast, abnormal outer dense fiber (ODF) structure was observed in <jats:italic>Tulp2<\/jats:italic> KO testis with TEM.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>TULP2 may play roles in the correct formation and\/or maintenance of ODF, which may lead to abnormal tail morphology, impaired sperm motility, and male infertility.<\/jats:p><\/jats:sec>","DOI":"10.1002\/rmb2.12467","type":"journal-article","created":{"date-parts":[[2022,5,23]],"date-time":"2022-05-23T19:55:05Z","timestamp":1653335705000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["TULP2 deletion mice exhibit abnormal outer dense fiber structure and male infertility"],"prefix":"10.1002","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4579-5487","authenticated-orcid":false,"given":"Yuki","family":"Oyama","sequence":"first","affiliation":[{"name":"Graduate School of Pharmaceutical Sciences Osaka 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