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Thus, analyses of the molecular processes of penile ED and continuous erection as priapism are essential issues of reproductive medicine.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>By performing mouse N\u2010ethyl\u2010N\u2010nitrosourea mutagenesis and exome sequencing, we established a novel mouse line displaying protruded genitalia phenotype (PGP; priapism\u2010like phenotype) and identified a novel <jats:italic>Pitpna<\/jats:italic> gene mutation for PGP. Extensive histological analyses on the <jats:italic>Pitpna<\/jats:italic> mutant and intracavernous pressure measurement (ICP) and liquid chromatography\u2013electrospray ionization tandem mass spectrometry (LC\u2013ESI\/MS)\/MS analyses were performed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We evaluated the role of phospholipids during erection for the first time and showed the mutants of inducible phenotypes of priapism. Moreover, quantitative analysis using LC\u2013ESI\/MS\/MS revealed that the level of phosphatidylinositol (PI) was significantly lower in the mutant penile samples. These results imply that PI may contribute to penile erection by PITP\u03b1.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our findings suggest that the current mutant is a mouse model for priapism and abnormalities in PI signaling pathways through PITP\u03b1 may lead to priapism providing an attractive novel therapeutic target in its treatment.<\/jats:p><\/jats:sec>","DOI":"10.1002\/rmb2.12472","type":"journal-article","created":{"date-parts":[[2022,6,20]],"date-time":"2022-06-20T07:41:18Z","timestamp":1655710878000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Establishment of mouse line showing inducible priapism\u2010like phenotypes"],"prefix":"10.1002","volume":"21","author":[{"given":"Daiki","family":"Hashimoto","sequence":"first","affiliation":[{"name":"Department of Developmental Genetics Institute of Advanced Medicine, Wakayama Medical University  Wakayama 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