{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T19:36:01Z","timestamp":1792870561921,"version":"4.5.0"},"reference-count":65,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,1,22]],"date-time":"2023-01-22T00:00:00Z","timestamp":1674345600000},"content-version":"vor","delay-in-days":21,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Medicine &amp; Biology"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Purpose<\/jats:title><jats:p>To clarify the mechanisms of intrauterine platelet\u2010rich plasma (PRP) infusion that support embryo implantation in in vitro fertilization treatment.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Blood and endometrial samples were collected from four infertile women. Human endometrial stromal cells (HESCs) were cultured and passaged equally into four cell culture dishes in each patient. Two were treated with PRP twice, and the other two were treated with vehicle. Subsequently, two cultures with and without PRP were decidualized with 8\u2010bromoadenosine 3\u2032,5\u2032\u2010cyclic AMP and progesterone for 5\u2009days.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The gene expression in undifferentiated or decidualized HESCs with and without PRP was compared. In the microarray analysis, 381 and 63 differentially expressed genes were detected in undifferentiated and decidualized HESCs, respectively. In the undifferentiated HESCs, PRP was found to promote the gene expression associated with cell growth, tissue regeneration, proinflammatory response, and antibiotic effects. In decidualized HESCs, PRP was found to attenuate the gene expression involved in cell proliferation and inflammation by inhibiting the expression of phosphoinositide 3\u2010kinase signaling.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Platelet\u2010rich plasma regulates the reprogramming of cell proliferation and inflammation depending on menstrual cycle phases in an appropriate manner, suggesting that PRP has the potential to increase endometrial thickness in the proliferative phase and improve immune tolerance in the secretory phase.<\/jats:p><\/jats:sec>","DOI":"10.1002\/rmb2.12498","type":"journal-article","created":{"date-parts":[[2023,1,23]],"date-time":"2023-01-23T05:59:10Z","timestamp":1674453550000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Transcriptomic profiling analysis of human endometrial stromal cells treated with autologous platelet\u2010rich plasma"],"prefix":"10.1002","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2759-9159","authenticated-orcid":false,"given":"Keiji","family":"Kuroda","sequence":"first","affiliation":[{"name":"Center for Reproductive Medicine and Endoscopy Sugiyama Clinic Marunouchi  Tokyo Japan"},{"name":"Centre for Reproductive Medicine and Implantation Research Sugiyama Clinic Shinjuku  Tokyo Japan"},{"name":"Department of Obstetrics and Gynaecology Juntendo University Faculty of Medicine  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akemi","family":"Matsumoto","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology Juntendo University Faculty of Medicine  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Horikawa","sequence":"additional","affiliation":[{"name":"Centre for Reproductive Medicine and Implantation Research Sugiyama Clinic Shinjuku  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1103-6856","authenticated-orcid":false,"given":"Satoru","family":"Takamizawa","sequence":"additional","affiliation":[{"name":"Centre for Reproductive Medicine and Implantation Research Sugiyama Clinic Shinjuku  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asako","family":"Ochiai","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology Juntendo University Faculty of Medicine  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuhiro","family":"Kawamura","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology Juntendo University Faculty of Medicine  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0874-5894","authenticated-orcid":false,"given":"Koji","family":"Nakagawa","sequence":"additional","affiliation":[{"name":"Centre for Reproductive Medicine and Implantation Research Sugiyama Clinic Shinjuku  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8892-2119","authenticated-orcid":false,"given":"Rikikazu","family":"Sugiyama","sequence":"additional","affiliation":[{"name":"Centre for Reproductive Medicine and Implantation Research Sugiyama Clinic Shinjuku  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,1,22]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2016.11.017"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2016.09.039"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jri.2017.10.045"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1210\/er.2014-1045"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dmu011"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.103.024109"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2012.05.004"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/des370"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.2174\/1574888X14666190213100528"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1080\/19396368.2017.1287225"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijms.22563"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm10194514"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.3109\/02713683.2015.1104362"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature07039"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI27196"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0030-1251502"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.3109\/09537104.2015.1116686"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1080\/19396368.2020.1862357"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tjog.2018.10.007"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/rmb2.12334"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1080\/09513590.2020.1756247"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1080\/09513590.2019.1597344"},{"issue":"1","key":"e_1_2_11_24_1","first-page":"1286","article-title":"Autologous platelet\u2010rich plasma promotes endometrial growth and improves pregnancy outcome during in vitro fertilization","volume":"8","author":"Chang Y","year":"2015","journal-title":"Int J Clin Exp Med"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.5935\/1518-0557.20170013"},{"issue":"1","key":"e_1_2_11_26_1","first-page":"42","article-title":"Platelet\u2010rich plasma as an adjuvant in the endometrial preparation of patients with refractory endometrium","volume":"22","author":"Molina A","year":"2018","journal-title":"JBRA Assist Reprod"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/aji.13376"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/rmb2.12412"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0173035"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.21769\/BioProtoc.2028"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2015.04.042"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0177387"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10815-018-1130-8"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1186\/scrt218"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12958-020-00637-5"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/gaq010"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2020.00105"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10815-021-02328-5"},{"issue":"2","key":"e_1_2_11_39_1","first-page":"113","article-title":"Platelet\u2010rich plasma in the management of Asherman's syndrome: an RCT","volume":"18","author":"Javaheri A","year":"2020","journal-title":"Int J Reprod Biomed"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10815-018-1135-3"},{"issue":"1","key":"e_1_2_11_41_1","first-page":"75","article-title":"Emerging principles for T cell receptor recognition of antigen in cellular immunity","volume":"1","author":"Garcia KC","year":"1999","journal-title":"Rev Immunogenet"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1111\/jog.13937"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1111\/aji.12719"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(13)60604-2"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.23736\/S0031\u20100808.20.04247\u20100"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.21873\/invivo.11504"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.31274"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-020-0763-1"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep03894"},{"key":"e_1_2_11_50_1","doi-asserted-by":"publisher","DOI":"10.1002\/uog.2742"},{"key":"e_1_2_11_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2019.05.001"},{"key":"e_1_2_11_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2019.12.030"},{"key":"e_1_2_11_53_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00255.2010"},{"key":"e_1_2_11_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2017.07.029"},{"key":"e_1_2_11_55_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.8.4240"},{"issue":"6","key":"e_1_2_11_56_1","first-page":"2334","article-title":"Changes in endometrial PTEN expression throughout the human menstrual cycle","volume":"85","author":"Mutter GL","year":"2000","journal-title":"J Clin Endocrinol Metab"},{"key":"e_1_2_11_57_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-019-1663-5"},{"key":"e_1_2_11_58_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.2921"},{"key":"e_1_2_11_59_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-53419-4"},{"key":"e_1_2_11_60_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.413583"},{"key":"e_1_2_11_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1471-4906(03)00139-X"},{"key":"e_1_2_11_62_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0897.2002.01105.x"},{"key":"e_1_2_11_63_1","doi-asserted-by":"publisher","DOI":"10.1111\/aji.13429"},{"key":"e_1_2_11_64_1","doi-asserted-by":"publisher","DOI":"10.4103\/tofj.tofj_11_18"},{"key":"e_1_2_11_65_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2020.e03577"},{"key":"e_1_2_11_66_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.15164"}],"container-title":["Reproductive Medicine and Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/rmb2.12498","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/rmb2.12498","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/rmb2.12498","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,2,22]],"date-time":"2024-02-22T17:21:59Z","timestamp":1708622519000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/rmb2.12498"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,1]]},"references-count":65,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2023,1]]}},"alternative-id":["10.1002\/rmb2.12498"],"URL":"https:\/\/doi.org\/10.1002\/rmb2.12498","archive":["Portico"],"relation":{},"ISSN":["1445-5781","1447-0578"],"issn-type":[{"value":"1445-5781","type":"print"},{"value":"1447-0578","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,1]]},"assertion":[{"value":"2022-11-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-12-23","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-01-22","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e12498"}}