{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T04:17:17Z","timestamp":1784953037594,"version":"3.55.0"},"reference-count":42,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,6,6]],"date-time":"2023-06-06T00:00:00Z","timestamp":1686009600000},"content-version":"vor","delay-in-days":156,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["22K09475"],"award-info":[{"award-number":["22K09475"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Medicine &amp; Biology"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Purpose<\/jats:title><jats:p>Spermatogenesis is a complex process orchestrated by several essential genes. <jats:italic>Prominin\u20101<\/jats:italic> (<jats:italic>Prom1<\/jats:italic>\/PROM1) is a gene that is expressed in the testis but with a poorly understood role in spermatogenesis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We used <jats:italic>Prom1<\/jats:italic> knockout (<jats:italic>Prom1<\/jats:italic> KO) mice to assess the role of <jats:italic>Prom1<\/jats:italic> in spermatogenesis. To this end, we performed immunohistochemistry, immunofluorescence, western blotting, \u03b2\u2010galactosidase staining, and apoptosis assay. Additionally, we analyzed the morphology of sperm and assessed litter sizes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We observed that PROM1 is localized to the dividing spermatocytes in seminiferous epithelial cells, sperm, and columnar epithelium in the epididymis. In the <jats:italic>Prom1<\/jats:italic> KO testis, an aberrant increase in apoptotic cells and a decrease in proliferating seminiferous epithelial cells were observed. Cellular FLICE\u2010like inhibitory protein (c\u2010FLIP) and extracellular signal\u2010regulated kinase 1\/2 (ERK1\/2) expression were also significantly decreased in <jats:italic>Prom1<\/jats:italic> KO testis. In addition, a significantly increased number of epididymal spermatozoa with abnormal morphology and less motility was found in <jats:italic>Prom1<\/jats:italic> KO mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>PROM1 maintains spermatogenic cell proliferation and survival via c\u2010FLIP expression in the testis. It is also involved in sperm motility and fertilization potential. The mechanism underlying the effect of Prom1 on sperm morphology and motility remains to be identified.<\/jats:p><\/jats:sec>","DOI":"10.1002\/rmb2.12514","type":"journal-article","created":{"date-parts":[[2023,6,8]],"date-time":"2023-06-08T03:13:09Z","timestamp":1686193989000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["<i>Prominin\u20101<\/i> deletion results in spermatogenic impairment, sperm morphological defects, and infertility in mice"],"prefix":"10.1002","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5634-6957","authenticated-orcid":false,"given":"Haruka","family":"Matsukuma","sequence":"first","affiliation":[{"name":"Department of Urology, School of Medicine Yamaguchi University  Ube Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuka","family":"Kobayashi","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, School of Medicine Yamaguchi 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