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From each sample, western blot (WB) and immunofluorescence (IF) analyses for RSPH6A were performed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>RSPH6A expression starts at 21 PND alongside the appearance of I spermatocytes (SPC) with a significant increase up to 60 PND. Data were confirmed by IF analysis, showing that RPSH6A expression is restricted to I and II SPC, spermatids, and mature sperm. <jats:italic>In vivo<\/jats:italic> experiments showed that the expression and localization of RSPH6A in the testis and epididymal spermatozoa of adult rats treated with cadmium were impaired. Interestingly, melatonin (an antioxidant), given together with Cd, can counteract its damaging effects.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>All combined data confirm that RSPH6A contributes to the onset of fertility by acting on sperm motility, raising the possibility of using RSPH6A as a marker for normal fertility in the general population.<\/jats:p><\/jats:sec>","DOI":"10.1002\/rmb2.12542","type":"journal-article","created":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T06:40:01Z","timestamp":1696315201000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Expression of <scp>RSPH6A<\/scp> in the first wave of rat spermatogenesis and oxidative stress conditions: Attenuation by melatonin"],"prefix":"10.1002","volume":"22","author":[{"given":"Maria Zelinda","family":"Romano","sequence":"first","affiliation":[{"name":"Dipartimento di Medicina Sperimentale, Sez. Fisiologia Umana e Funzioni Biologiche Integrate \u201cF. Bottazzi\u201d Universit\u00e0 degli Studi della Campania \u201cLuigi Vanvitelli\u201d  Napoli Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariem","family":"Ben\u00a0Rhouma","sequence":"additional","affiliation":[{"name":"Laboratoire LR11ES41 G\u00e9n\u00e9tique Biodiversit\u00e9 et Valorisation des Bio\u2010Ressourc\u00e9s Institut Sup\u00e9rieur de Biotechnologie de Monastir, Universit\u00e9 de Monastir  Monastir Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Imed","family":"Messaoudi","sequence":"additional","affiliation":[{"name":"Laboratoire LR11ES41 G\u00e9n\u00e9tique Biodiversit\u00e9 et Valorisation des Bio\u2010Ressourc\u00e9s Institut Sup\u00e9rieur de Biotechnologie de Monastir, Universit\u00e9 de Monastir  Monastir Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francesco","family":"Aniello","sequence":"additional","affiliation":[{"name":"Dipartimento di Biologia Universit\u00e0 di Napoli \u201cFederico II\u201d  Napoli Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1007-7840","authenticated-orcid":false,"given":"Sergio","family":"Minucci","sequence":"additional","affiliation":[{"name":"Dipartimento di Medicina Sperimentale, Sez. 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