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This study was conducted to optimize the cultural environment.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We used 35 blastocysts that had been discarded after in\u2010vitro fertilization. The concordance rate of karyotype analysis results between whole embryos (WEs), spent culture mediums (SCMs), and trophectoderms after 8, 16, and 24\u2009h of culture was examined. Next, zona pellucida (ZP)\u2010free blastocysts and then early blastocysts were cultured for 24\u2009h each.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Regarding the optimal culture times, the concordance rate between WEs and SCMs was 20%, 60%, and 100% at 8, 16, and 24\u2009h, respectively. Significant differences were found between 8 and 24\u2009h. The concordance rate with ZP cultures was 40.0%, and no significant differences were found. The concordance rate of early blastocysts thawed and cultured for 24\u2009h was 40.0%, which was significantly lower than that of day 5 blastocysts.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The optimal culture times for niPGT\u2010A were 24\u2009h, and the concordance rate with free ZP was higher. The concordance rate for early blastocysts was low, suggesting that optimization of the conditions may be necessary.<\/jats:p><\/jats:sec>","DOI":"10.1002\/rmb2.12604","type":"journal-article","created":{"date-parts":[[2024,9,11]],"date-time":"2024-09-11T06:01:40Z","timestamp":1726034500000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Conditions for improved accuracy of noninvasive preimplantation genetic testing for aneuploidy: Focusing on the zona pellucida and early blastocysts"],"prefix":"10.1002","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5994-7416","authenticated-orcid":false,"given":"Hiroki","family":"Takeuchi","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology, Graduate School of Medicine Mie University  Tsu Japan"},{"name":"Center of Advanced Reproductive Medicine Mie University Hospital  Tsu 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