{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T10:10:02Z","timestamp":1753870202155,"version":"3.41.2"},"reference-count":14,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,9,23]],"date-time":"2024-09-23T00:00:00Z","timestamp":1727049600000},"content-version":"vor","delay-in-days":266,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["19K09665","22K09518"],"award-info":[{"award-number":["19K09665","22K09518"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Medicine &amp; Biology"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Purpose<\/jats:title><jats:p>The purpose of this study is to confirm whether in\u00a0vitro fertilization (IVF) with spermatozoa from <jats:italic>Odf4<\/jats:italic>\u2010deficient infertile males (<jats:italic>Odf4<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> spermatozoa) can lead to the development of zygotes, which was reported in a previous in\u00a0vivo study.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In vitro capacitation and IVF were performed using <jats:italic>Odf4<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> spermatozoa in a small drop of TYH medium with pyruvate and glucose, for 60\u2009min or up to 4\u2009days. A capacitation test was performed by immunoblotting using an anti\u2010p\u2010Tyr antibody. A sperm movement test was performed using a computer\u2010assisted sperm motility analysis system (SMAS). An IVF fertilization test was also performed to evaluate zygote production. Videos were taken by a DMi8 stereomicroscope equipped with a high\u2010speed camera.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In in\u00a0vitro condition, <jats:italic>Odf4<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> spermatozoa with hairpin flagella harboring large cytoplasmic droplets (CDs) underwent capacitation, about 30% of large CDs were removed from spermatozoa, and the flagella became straight (capacitation test). The <jats:italic>Odf4<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> spermatozoa with straight flagella swam forward (movement test) and fertilized <jats:italic>Odf4<\/jats:italic><jats:sup>+\/+<\/jats:sup> oocytes, which eventually developed into zygotes (fertilization test).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>By conventional IVF, spermatozoa from <jats:italic>Odf4<\/jats:italic>\u2010deficient male mice can fertilize oocytes that then develop into zygotes. These findings can be translated to human males with infertility caused by <jats:italic>ODF4<\/jats:italic> deficiency.<\/jats:p><\/jats:sec>","DOI":"10.1002\/rmb2.12605","type":"journal-article","created":{"date-parts":[[2024,9,23]],"date-time":"2024-09-23T15:43:28Z","timestamp":1727106208000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Spermatozoa from male mice with infertility due to <i>Odf4<\/i> deficiency can fertilize oocytes by in\u00a0vitro fertilization"],"prefix":"10.1002","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2697-0382","authenticated-orcid":false,"given":"Chizuru","family":"Ito","sequence":"first","affiliation":[{"name":"Department of Functional Anatomy, Graduate School of Medicine Chiba University  Chiba Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tohru","family":"Mutoh","sequence":"additional","affiliation":[{"name":"Department of Functional Anatomy, Graduate School of Medicine Chiba University  Chiba Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-8743-1263","authenticated-orcid":false,"given":"Kiyotaka","family":"Toshimori","sequence":"additional","affiliation":[{"name":"Department of Functional Anatomy, Graduate School of Medicine Chiba University  Chiba Japan"},{"name":"Future Medicine Research Center Chiba University  Chiba Japan"},{"name":"Chiba University  Chiba Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,9,23]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"99","volume-title":"Knobil and Neill's physiology of reproduction. 1","author":"Toshimori K","year":"2014"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10815\u2010022\u201002516\u2010x"},{"volume-title":"WHO laboratory manual for the Examination and processing of human semen","year":"2021","author":"World Health Organization","key":"e_1_2_8_4_1"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010023\u201028177\u2010z"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10815\u2010022\u201002516\u2010x"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms10041729"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2014.01.014"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.106.053512"},{"key":"e_1_2_8_10_1","first-page":"149","volume-title":"Knobil and Neill's physiology of reproduction. 1","author":"Florman HM","year":"2014"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1274\/jmor.33.3"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.121.4.1129"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010019\u201050516\u20102"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP\u201018\u20100394"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.113.114447"}],"container-title":["Reproductive Medicine and Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/rmb2.12605","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,6]],"date-time":"2025-01-06T14:42:45Z","timestamp":1736174565000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/rmb2.12605"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,1]]},"references-count":14,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2024,1]]}},"alternative-id":["10.1002\/rmb2.12605"],"URL":"https:\/\/doi.org\/10.1002\/rmb2.12605","archive":["Portico"],"relation":{},"ISSN":["1445-5781","1447-0578"],"issn-type":[{"type":"print","value":"1445-5781"},{"type":"electronic","value":"1447-0578"}],"subject":[],"published":{"date-parts":[[2024,1]]},"assertion":[{"value":"2024-04-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-08-14","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-09-23","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e12605"}}