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Despite the transfer of high\u2010quality blastocysts, the implantation rate of IVF\u2010derived blastocysts remains low after ET.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>This article provides a comprehensive review of current research on embryo implantation regulators and their application to improve the implantation potential of IVF\u2010derived blastocysts.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Main Findings<\/jats:title>\n                    <jats:p>\n                      The in\u00a0vivo mouse model revealed selective proteolysis immediately after expression in activated blastocysts, that is, degradation of ER\u03b1 expression in activated blastocysts regulated by the ubiquitin\u2010proteasome pathway, followed by completion of blastocyst implantation. Treatment of blastocysts to induce appropriate protein expression during in\u00a0vitro culture prior to ET is a useful approach for improving implantation rates. This approach showed that combined treatment with PRL, EGF, and 4\u2010OH\u2010E\n                      <jats:sub>2<\/jats:sub>\n                      (PEC) improved the blastocyst implantation rates. Furthermore, arginine and leucine drive reactive oxygen species (ROS)\u2010mediated integrin \u03b15\u03b21 expression and promote blastocyst implantation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Findings based on analysis of molecular and cellular regulators are useful for improving the implantation potential of IVF\u2010derived blastocysts. These approaches may help to elucidate the mechanisms underlying the completion of the blastocyst implantation, although further investigation is required to improve the success of implantation and pregnancy.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rmb2.12633","type":"journal-article","created":{"date-parts":[[2025,1,24]],"date-time":"2025-01-24T05:36:17Z","timestamp":1737696977000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Molecular and cellular regulators of embryo implantation and their application in improving the implantation potential of\n                    <scp>IVF<\/scp>\n                    \u2010derived blastocysts"],"prefix":"10.1002","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5762-2036","authenticated-orcid":false,"given":"Chunyan","family":"Liu","sequence":"first","affiliation":[{"name":"Laboratory of Animal Breeding and Reproduction, Division of Animal 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