{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T19:13:13Z","timestamp":1789240393624,"version":"build-2803163510"},"reference-count":42,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,3,19]],"date-time":"2025-03-19T00:00:00Z","timestamp":1742342400000},"content-version":"vor","delay-in-days":77,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Medicine &amp; Biology"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Purpose<\/jats:title>\n                    <jats:p>Testis is one of the most radiosensitive tissues. Interstitial stromal cells play a supportive role in male fertility, but radiation\u2010induced damage to those cells has not yet been well understood. We aimed to investigate radiation\u2010induced changes in interstitial stromal cells in the testis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Adult male C57BL\/6N mice (8\u2009weeks) received a single pelvic exposure to a relatively high dose (1 Gy) or a very high dose (8 Gy) X\u2010ray. We collected the testicular tissues for evaluation at 1, 9, and 60\u2009days after irradiation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>We detected a recoverable moderate degeneration of seminiferous tubules after 1\u2009Gy exposure but an irreversible severe damage to the testis after 8 Gy exposure. Immunostaining results revealed that 1\u2009Gy exposure induced DNA damage at day 1, upregulated intratubular GDNF at days 1 and 9, upregulated FGF at all time points, and upregulated CSF\u20101R at day 9. In contrast, 8\u2009Gy exposure induced DNA damage at days 1 and 9, upregulated intratubular GDNF at days 1 and 9, downregulated CD105 at day 60, and upregulated FGF at all time points.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Radiation\u2010induced dynamic changes to interstitial stromal cells in the testis. Upregulated interstitial CSF\u20101R and FGF2 may support spermatogenesis recovery after high\u2010dose radiation.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rmb2.12639","type":"journal-article","created":{"date-parts":[[2025,3,19]],"date-time":"2025-03-19T06:37:39Z","timestamp":1742366259000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["The effect of ionizing radiation on testicular interstitial stromal cells"],"prefix":"10.1002","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-3580-5685","authenticated-orcid":false,"given":"Esraa","family":"Tamam","sequence":"first","affiliation":[{"name":"Department of Stem Cell Biology, Atomic Bomb Disease Institute, Graduate School of Biomedical Sciences Nagasaki University  Nagasaki Japan"},{"name":"Department of Anatomy and Embryology, Faculty of Veterinary Medicine Sohag University  Sohag Governate 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