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The expression of microRNA miR\u201029c\u20103p is dysregulated in endometriosis. We aimed to identify novel molecular targets of miR\u201029c\u20103p functionally linked to proliferation and invasive growth in endometriosis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The epithelial endometriotic cell line 12Z and primary endometriotic stromal cells (PESC) were transfected with control miRNA or pre\u2010miR\u201029c\u20103p, and subjected to cell cycle analysis, cell viability, wound healing, and Matrigel invasion assays. Expression of bioinformatically predicted miR\u201029c\u20103p targets was analyzed by qPCR and western blot. Target gene expression in endometriotic lesions and healthy endometrium was studied in the EndometDB endometriosis database.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      miR\u201029c\u20103p decreased 12Z and PESC cell viability and the proportion of PESC in the S\u2010phase. 12Z cell invasion, but not migration, was decreased after miR\u201029c\u20103p upregulation. miR\u201029c\u20103p decreased the mRNA expression of\n                      <jats:italic>CDK6<\/jats:italic>\n                      ,\n                      <jats:italic>BCCIP<\/jats:italic>\n                      ,\n                      <jats:italic>TCF7L1<\/jats:italic>\n                      ,\n                      <jats:italic>TCF7L2<\/jats:italic>\n                      ,\n                      <jats:italic>PTEN<\/jats:italic>\n                      ,\n                      <jats:italic>COL4A1<\/jats:italic>\n                      ,\n                      <jats:italic>E\u2010Cadherin<\/jats:italic>\n                      , and\n                      <jats:italic>N\u2010Cadherin<\/jats:italic>\n                      . A decrease of CDK6 and PTEN and an increase of p21 were confirmed at the protein level. EndometDB database analysis demonstrated dysregulated expression of the selected targets in both deep endometriosis and ovarian endometriosis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>miR\u201029c\u20103p effectively curbs endometriotic cell proliferation and invasion by combined inhibition of cell cycle regulators and transcription factors, unveiling a promising therapeutic strategy.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rmb2.12645","type":"journal-article","created":{"date-parts":[[2025,3,27]],"date-time":"2025-03-27T00:37:02Z","timestamp":1743035822000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Combined targeting of\n                    <scp>TCF7L1<\/scp>\n                    \/2,\n                    <scp>PTEN<\/scp>\n                    ,\n                    <scp>CDK6<\/scp>\n                    , and\n                    <scp>BCCIP<\/scp>\n                    by\n                    <scp>microRNA miR<\/scp>\n                    \u201029c\u20103p is associated with reduced invasion and proliferation of endometriotic cells"],"prefix":"10.1002","volume":"24","author":[{"given":"Teresa Helene","family":"Wentges","sequence":"first","affiliation":[{"name":"Department of Gynecology and Obstetrics M\u00fcnster University Hospital, Research Laboratory  M\u00fcnster Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heba M.","family":"El\u2010Shorafa","sequence":"additional","affiliation":[{"name":"Department of Gynecology and Obstetrics M\u00fcnster University Hospital, Research Laboratory  M\u00fcnster Germany"},{"name":"Department of Laboratory Medical Sciences, Faculty of Medical Sciences Alaqsa University  Gaza 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