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However, its role in spermatogenesis remains unclear. Recent transcriptomic analyses suggest a potential link between KIF2C and male infertility. This study aimed to clarify KIF2C's roles in spermatogenesis using\n                      <jats:italic>Kif2c<\/jats:italic>\n                      knockout (KO) mice.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      To overcome the preweaning lethality associated with\n                      <jats:italic>Kif2c<\/jats:italic>\n                      deletion, we generated\n                      <jats:italic>Kif2c<\/jats:italic>\n                      KO mice with a mixed genetic background of 129X1\/SvJ and B6D2. We assessed male fertility, epididymal sperm counts, and testicular sections of\n                      <jats:italic>Kif2c<\/jats:italic>\n                      KO mice.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Global\n                      <jats:italic>Kif2c<\/jats:italic>\n                      KO mice were obtained and showed male infertility. Histological analyses and epididymal sperm count revealed that\n                      <jats:italic>Kif2c<\/jats:italic>\n                      KO mice exhibited severely impaired spermatogenesis and absence of mature spermatozoa. These findings are consistent with those observed in patients with nonobstructive azoospermia (NOA). Our classification of\n                      <jats:italic>Kif2c<\/jats:italic>\n                      KO seminiferous tubules indicated that most spermatogenic cells were arrested at the early stages, particularly during meiosis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      This study provides in\u00a0vivo evidence that KIF2C is essential for spermatogenesis and male fertility in mice. The successful generation of global\n                      <jats:italic>Kif2c<\/jats:italic>\n                      KO mice establishes an animal model for NOA, supporting research on germ cell development and reproductive health.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rmb2.12659","type":"journal-article","created":{"date-parts":[[2025,5,27]],"date-time":"2025-05-27T09:00:39Z","timestamp":1748336439000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["<scp>KIF2C<\/scp>\n                    Deletion Causes Meiotic Abnormalities and Nonobstructive Azoospermia in Mice"],"prefix":"10.1002","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-8421-7911","authenticated-orcid":false,"given":"Hiroaki","family":"Kitakaze","sequence":"first","affiliation":[{"name":"Research Institute for Microbial Diseases The University of Osaka  Osaka Japan"},{"name":"Department of 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