{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T01:47:16Z","timestamp":1785376036887,"version":"3.55.0"},"reference-count":51,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,6,23]],"date-time":"2025-06-23T00:00:00Z","timestamp":1750636800000},"content-version":"vor","delay-in-days":173,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100007219","name":"Natural Science Foundation of Shanghai Municipality","doi-asserted-by":"publisher","award":["19ZR1476100"],"award-info":[{"award-number":["19ZR1476100"]}],"id":[{"id":"10.13039\/100007219","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Medicine &amp; Biology"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Purpose<\/jats:title>\n                    <jats:p>Polycystic ovary syndrome (PCOS) is an endocrine syndrome that afflicts women of childbearing age, whose specific pathogenesis is unknown. Combined multiomics analysis on it is still lacking. The purpose of this study was to use combined multiomics analyses to learn about the development of PCOS.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We randomly selected three PCOS mouse models and two control mice as the mouse group, as well as three PCOS patients and two normal women as the human group. The data were analyzed by multiomics analysis including methylomes, transcriptomics, and metabolomics. We explored the key genes involved in the occurrence and development of PCOS that were common in multiomics. Methylation\u2010specific polymerase chain reaction (MSP) and real\u2010time PCR (qPCR) experiments were performed to verify the reliability of the results.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The gene\n                      <jats:italic>ANXA1<\/jats:italic>\n                      was hypomethylated and highly expressed in both mouse and human samples. Meanwhile,\n                      <jats:italic>LDLR<\/jats:italic>\n                      had a lower expression in both mouse and human samples, targeted by an upregulated microRNA (miRNA) called has\u2010miR\u2010106a\u20105p, which may relate to hyperandrogenemia.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      Epigenetic mechanisms have an impact on the development of PCOS. Both\n                      <jats:italic>ANXA1<\/jats:italic>\n                      and\n                      <jats:italic>LDLR<\/jats:italic>\n                      play important roles in the pathological process of PCOS and have the potential to be diagnostic markers and therapeutic targets.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rmb2.12664","type":"journal-article","created":{"date-parts":[[2025,6,23]],"date-time":"2025-06-23T06:15:19Z","timestamp":1750659319000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Combined Multiomics Analysis Reveals the Role of\n                    <scp>\n                      <i>ANXA1<\/i>\n                    <\/scp>\n                    Methylation and\n                    <scp>miRNA<\/scp>\n                    \u2010Targeted\n                    <scp>\n                      <i>LDLR<\/i>\n                    <\/scp>\n                    in Polycystic Ovary Syndrome"],"prefix":"10.1002","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-6151-9679","authenticated-orcid":false,"given":"Yicen","family":"Ding","sequence":"first","affiliation":[{"name":"School of Biomedical Engineering, Bio\u2010ID Center Shanghai Jiao Tong University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lishi","family":"Huang","sequence":"additional","affiliation":[{"name":"Peace Maternity and Child Health Hospital School of Medicine, Shanghai Jiao Tong University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengju","family":"He","sequence":"additional","affiliation":[{"name":"School of Biomedical Engineering, Bio\u2010ID Center Shanghai Jiao Tong University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Biomedical Engineering, Bio\u2010ID Center Shanghai Jiao Tong University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanjun","family":"Jiang","sequence":"additional","affiliation":[{"name":"School of Biomedical Engineering, Bio\u2010ID Center Shanghai Jiao Tong University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yani","family":"Kang","sequence":"additional","affiliation":[{"name":"School of Biomedical Engineering, Bio\u2010ID Center Shanghai Jiao Tong University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,6,23]]},"reference":[{"key":"e_1_2_10_2_1","volume-title":"Polycystic Ovary Syndrome","author":"Health OoWs","year":"2021"},{"key":"e_1_2_10_3_1","volume-title":"Polycystic Ovary Syndrome","author":"Organization 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