{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T20:52:16Z","timestamp":1769633536326,"version":"3.49.0"},"reference-count":71,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T00:00:00Z","timestamp":1760486400000},"content-version":"vor","delay-in-days":287,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Medicine &amp; Biology"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Purpose<\/jats:title>\n                    <jats:p>\n                      This study investigated the therapeutic effects of Chitosan\/Alginate\u2010loaded\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>Astragalus hamosus<\/jats:italic>\n                      <\/jats:styled-content>\n                      extract (Chn\/Al\u2010AH) on the expression of\n                      <jats:italic>miRNA\u2010222<\/jats:italic>\n                      and\n                      <jats:italic>ESR1<\/jats:italic>\n                      genes, inflammatory cytokines, lipid profile, and reproductive hormones in a rat model of polycystic ovary syndrome (PCOS) induced by estradiol valerate (EV).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Twenty\u2010five female Wistar rats, weighing an average of 180\u2009g, were divided into control and PCOS groups. The PCOS model was induced by a single intramuscular injection of EV (4\u2009mg\/kg). After 28\u2009days of PCOS induction, the rats were orally administered Chitosan\/Alginate\u2010loaded AH at 5, 10, and 15\u2009mg\/kg. Following 4\u2009weeks of treatment, histological and biochemical parameters, pro\u2010inflammatory cytokines, body weight, and the expression of miRNA\u2010222 and ESR1 genes were evaluated.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      EV\u2010induced PCOS rats showed increased body weight, dyslipidemia, elevated inflammatory cytokines, disrupted sex hormone levels, upregulation of\n                      <jats:italic>miRNA\u2010222<\/jats:italic>\n                      , and decreased\n                      <jats:italic>ESR1<\/jats:italic>\n                      expression. Treatment with Chn\/Al\u2010AH significantly ameliorated these alterations by improving metabolic and endocrine profiles, reducing\n                      <jats:italic>miRNA\u2010222<\/jats:italic>\n                      expression, enhancing follicular development, decreasing cystic follicles, and promoting corpus luteum formation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Chn\/Al\u2010AH demonstrates therapeutic potential in mitigating hormonal, inflammatory, and genetic disruptions associated with PCOS in rats.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rmb2.12679","type":"journal-article","created":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T19:35:38Z","timestamp":1760556938000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Chitosan\/Alginate\u2010Loaded\n                    <scp>\n                      <i>Astragalus hamosus<\/i>\n                    <\/scp>\n                    Shows Ameliorative Effects on Lipid Profile, Inflammatory and Hormonal Parameters, and Reduces\n                    <scp>miRNA<\/scp>\n                    \u2010222 Expression in Polycystic Ovary Syndrome Rats"],"prefix":"10.1002","volume":"24","author":[{"given":"Rashid","family":"Jafardoust","sequence":"first","affiliation":[{"name":"Department of Biology, Ur.C Islamic Azad University  Urmia Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8285-5699","authenticated-orcid":false,"given":"Maryam","family":"Parvini Kohneh Shahri","sequence":"additional","affiliation":[{"name":"Department of Biology, Ur.C Islamic Azad University  Urmia Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zohreh","family":"Ghazi Tabatabaei","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Ah.C Islamic Azad University  Ahar Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ata","family":"Pouryaee","sequence":"additional","affiliation":[{"name":"Department of Biology, Ur.C Islamic Azad University  Urmia Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,10,15]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2015.12.013"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/rmb2.12630"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13668-023-00479-8"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2022.892125"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/rmb2.12487"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2023.e13024"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2024.113859"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jri.2022.103628"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00404-020-05951-2"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells12070983"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/genes5030684"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2013-2218"},{"key":"e_1_2_10_14_1","first-page":"918","article-title":"MiR\u2010222 Promotes the Progression of Polycystic Ovary Syndrome by Targeting p27 Kip1","volume":"215","author":"Huang X.","year":"2019","journal-title":"Pathology"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2022.12.045"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00404-024-07379-4"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1155\/2023\/3705508"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/biotech12010004"},{"key":"e_1_2_10_19_1","first-page":"1","article-title":"Therapeutics, Phytochemistry and Pharmacology of Iklilul Malik (Astragalus hamosus Linn): A Natural Unani Remedy","volume":"5","author":"Umer K. 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