{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T20:59:01Z","timestamp":1769633941294,"version":"3.49.0"},"reference-count":50,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T00:00:00Z","timestamp":1760486400000},"content-version":"vor","delay-in-days":287,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Medicine &amp; Biology"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Purpose<\/jats:title>\n                    <jats:p>Recurrent spontaneous abortion (RSA) is an abnormal phenomenon that severely affects women's quality of life. Inhibiting Th17 cell differentiation can alleviate RSA. This research explored the mechanism by which BRD4 Inhibitor (BETi) suppressed the differentiation of Th17 cells to mitigate RSA.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      PBMCs and Naive CD4\n                      <jats:sup>+<\/jats:sup>\n                      T cells were induced to differentiate into Th17 and Treg cells. An abortion\u2010prone pregnancy mouse model was constructed by intraperitoneal injection of lipopolysaccharide. The Th17\/Treg ratio was determined by flow cytometry. The association between STAT3 and IL\u201017A promoter was investigated by ChIP and dual luciferase assays. Co\u2010IP and yeast two\u2010hybrid assays were used to determine BRD4 binding to STAT3. The markers of Th17\/Treg cell differentiation and lipid synthesis were checked by ELISA, IHC, RT\u2010qPCR, and Western blot.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The Th17\/Treg ratio and the expression levels of BRD4, STAT3, and IL\u201017A were elevated, while STAT5b expression was down\u2010regulated in RSA patients. BETi or STAT3 knockdown decreased the differentiation of Th17 cells and lipid synthesis. BRD4 inhibition impaired STAT3\u2010mediated IL\u201017A transcription. BETi inhibited embryo absorption in mice.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>BETi inhibits the differentiation of Th17 cells in RSA by reducing the STAT3\u2010mediated IL\u201017A expression.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rmb2.12682","type":"journal-article","created":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T19:35:51Z","timestamp":1760556951000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>BRD4<\/scp>\n                    Inhibitor Alleviates Recurrent Spontaneous Abortion via Regulating\n                    <scp>BRD4<\/scp>\n                    \/\n                    <scp>STAT3<\/scp>\n                    \/\n                    <scp>IL<\/scp>\n                    \u2010\n                    <scp>17A<\/scp>\n                    Axis to Decrease the Th17 Cell Differentiation"],"prefix":"10.1002","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7377-925X","authenticated-orcid":false,"given":"Fen","family":"Liu","sequence":"first","affiliation":[{"name":"The Obstetrics and Gynecology Department The First Hospital of Changsha  Changsha China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhenhui","family":"Zhang","sequence":"additional","affiliation":[{"name":"The Obstetrics and Gynecology Department The First Hospital of Changsha  Changsha China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chengying","family":"Yang","sequence":"additional","affiliation":[{"name":"The Obstetrics and Gynecology Department The First Hospital of Changsha  Changsha 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