{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T21:33:47Z","timestamp":1769636027739,"version":"3.49.0"},"reference-count":38,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,11,14]],"date-time":"2025-11-14T00:00:00Z","timestamp":1763078400000},"content-version":"vor","delay-in-days":317,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100022077","name":"Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100022077","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Medicine &amp; Biology"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Purpose<\/jats:title>\n                    <jats:p>\n                      The influence of culture media used during in\u00a0vitro fertilization (IVF) on offspring phenotype remains controversial. However, specific effects of short exposure time after fertilization remain underexplored. By evaluating Ca\n                      <jats:sup>2+<\/jats:sup>\n                      oscillations as a readout of the first response of eggs to their microenvironment, we aim to investigate if early differences correlate with later adult phenotypes.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Oocytes fertilized by ICSI were cultured for 4\u2009h in three different media (Cook and Vitrolife, used in human IVF, and KSOM, used for mouse embryos). They were either measured for Ca\n                      <jats:sup>2+<\/jats:sup>\n                      oscillations or transferred into pseudo\u2010pregnant females. After birth, growth curves of pups were measured up to adulthood and various organs were weighed.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Culture media significantly modulate Ca\n                      <jats:sup>2+<\/jats:sup>\n                      oscillations during oocyte activation. ICSI\u2010fertilized oocytes cultured in Cook and Vitrolife exhibited fewer oscillations, lower frequency, and reduced variability compared to KSOM. These early differences correlated with long\u2010term developmental outcomes: females from Cook and Vitrolife cultures were heavier throughout growth and had larger adult organ sizes compared to those from KSOM.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      Brief exposure to media immediately after ICSI shapes Ca\n                      <jats:sup>2+<\/jats:sup>\n                      dynamics and adult phenotypes. Optimizing embryo culture protocols in assisted reproductive technologies may improve IVF outcomes by modulating metabolic pathways linked to development.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rmb2.12691","type":"journal-article","created":{"date-parts":[[2025,11,14]],"date-time":"2025-11-14T14:43:48Z","timestamp":1763131428000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Short\u2010Term Exposure to Culture Media Used in Human\n                    <scp>ART<\/scp>\n                    Shapes Early Calcium Oscillations in\n                    <scp>ICSI<\/scp>\n                    \u2010Fertilized Mouse Oocytes and Impacts Adult Phenotype"],"prefix":"10.1002","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-9169-8188","authenticated-orcid":false,"given":"Bernadette","family":"Banrezes","sequence":"first","affiliation":[{"name":"UVSQ, INRAE, BREED, Universit\u00e9 Paris\u2010Saclay  Jouy\u2010en\u2010Josas France"},{"name":"Ecole Nationale V\u00e9t\u00e9rinaire d'Alfort, BREED  Maisons\u2010Alfort France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-9374-5196","authenticated-orcid":false,"given":"Thierry","family":"Sainte\u2010Beuve","sequence":"additional","affiliation":[{"name":"UVSQ, INRAE, BREED, Universit\u00e9 Paris\u2010Saclay  Jouy\u2010en\u2010Josas France"},{"name":"Ecole Nationale V\u00e9t\u00e9rinaire d'Alfort, BREED  Maisons\u2010Alfort France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4696-5011","authenticated-orcid":false,"given":"Anne","family":"Frambourg","sequence":"additional","affiliation":[{"name":"UVSQ, INRAE, BREED, Universit\u00e9 Paris\u2010Saclay  Jouy\u2010en\u2010Josas France"},{"name":"Ecole Nationale V\u00e9t\u00e9rinaire d'Alfort, BREED  Maisons\u2010Alfort France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5985-9862","authenticated-orcid":false,"given":"Alice","family":"Jouneau","sequence":"additional","affiliation":[{"name":"UVSQ, INRAE, BREED, Universit\u00e9 Paris\u2010Saclay  Jouy\u2010en\u2010Josas France"},{"name":"Ecole Nationale V\u00e9t\u00e9rinaire d'Alfort, BREED  Maisons\u2010Alfort France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,11,14]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dead197"},{"key":"e_1_2_10_3_1","volume-title":"State\u2010Specific Assisted Reproductive Technology Surveillance, United States: 2019 Data Brief","author":"Sunderam S.","year":"2021"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.15585\/mmwr.ss7104a1"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.01181"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2024.1474009"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/deu346"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10815-023-02934-5"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dep456"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/des145"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/deu025"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dey246"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2013.07.006"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.4414\/smw.2014.14038"},{"issue":"2","key":"e_1_2_10_15_1","first-page":"138","article-title":"Randomized Comparison of Two Commercial Culture Media (Cook and Vitrolife) for Embryo Culture After IMSI","volume":"23","author":"Petersen C. G.","year":"2019","journal-title":"JBRA Assisted Reproduction"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2022.884088"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0306846101"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0029388"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP-16-0057"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(18)30312-X"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-66019-4"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2023.107321"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2014.05.043"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2017.01.007"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/deae248"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dez277"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.128.6.917"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2005.02.035"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1530\/rep.1.00764"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(93)25012-Q"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1995.sp020589"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2006.02.010"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.301.6761.1111"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.129.15.3533"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/316541a0"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells12242809"},{"issue":"44","key":"e_1_2_10_37_1","first-page":"E10370","article-title":"TRPM7 and CaV 3.2 Channels Mediate Ca2+ Influx Required for Egg Activation at Fertilization","volume":"115","author":"Bernhardt M. L.","year":"2018","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep34236"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1101\/2025.03.14.643362"}],"container-title":["Reproductive Medicine and Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/rmb2.12691","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/rmb2.12691","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/rmb2.12691","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T08:54:37Z","timestamp":1769590477000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/rmb2.12691"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1]]},"references-count":38,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,1]]}},"alternative-id":["10.1002\/rmb2.12691"],"URL":"https:\/\/doi.org\/10.1002\/rmb2.12691","archive":["Portico"],"relation":{},"ISSN":["1445-5781","1447-0578"],"issn-type":[{"value":"1445-5781","type":"print"},{"value":"1447-0578","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1]]},"assertion":[{"value":"2025-04-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-21","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-14","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e12691"}}