{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T11:55:56Z","timestamp":1789646156607,"version":"4.0.0"},"reference-count":35,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T00:00:00Z","timestamp":1769558400000},"content-version":"vor","delay-in-days":27,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2019YFC1709500"],"award-info":[{"award-number":["2019YFC1709500"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Medicine &amp; Biology"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Purpose<\/jats:title>\n                    <jats:p>To compare total testosterone (TT) measured by liquid chromatograph\u2010tandem mass spectrometry (LC\u2013MS\/MS) with electro\u2010chemiluminescent immunoassay (ECLIA) in the diagnosis and management of infertile women with polycystic ovary syndrome (PCOS).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Baseline TT was measured by LC\u2013MS\/MS and ECLIA in 906 infertile women with PCOS. The associations of TT from both methods with clinical phenotypes and fertility outcomes were estimated; relative risk (RR) and 95% confidence intervals (CIs) were computed. Subgroup analysis was conducted according to the TT levels.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The average TT levels measured by ECLIA were higher than those measured by LC\u2013MS\/MS (mean percentage difference 23.8%, 95% limits of agreement \u221244.2% to 91.9%). When biochemical hyperandrogenism (HA) defined as TT \u2265\u20091.7\u2009nmol\/L by LC\u2013MS\/MS method, a higher proportion of patients were identified having biochemical HA using ECLIA (44.0% vs. 24.0%,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001) than LC\u2013MS\/MS. Only those with TT levels \u2265\u20091.7\u2009nmol\/L measured by LC\u2013MS\/MS had an increased risk of adverse fertility outcomes compared to patients with normal TT levels, including ovulation, preterm labor, and neonatal intensive care unit.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Our findings indicated that LC\u2013MS\/MS refined the diagnosis of biochemical hyperandrogenism and better identified the subgroup at genuine risk of adverse fertility outcomes in infertile women with PCOS.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Trial Registration<\/jats:title>\n                    <jats:p>The NIH Clinical Trial Registry number: NCT01573858 and Chinese Clinical Trial. Registry number: ChiCTR\u2010TRC\u201012002081<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rmb2.70013","type":"journal-article","created":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T11:28:43Z","timestamp":1769599723000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Total Testosterone Measured by Liquid Chromatograph\u2010Tandem Mass Spectrometry Refines Diagnosis of Biochemical Hyperandrogenism and Better Identifies Subgroup at Genuine Risk of Adverse Fertility Outcomes in Women With Polycystic Ovary Syndrome"],"prefix":"10.1002","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1555-4327","authenticated-orcid":false,"given":"Jian","family":"Li","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology, Affiliated Hospital Guizhou Medical University  Guiyang China"},{"name":"Department of Obstetrics and Gynecology, First Affiliated Hospital Heilongjiang University of Chinese Medicine  Harbin China"},{"name":"Department of Obstetrics and Gynaecology The Chinese University of Hong Kong  Hong Kong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology The Chinese University of Hong Kong  Hong Kong China"},{"name":"Department of Research and Development Shenzhen Coreion Biotechnology Co., Ltd  Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Cong","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, First Affiliated Hospital Heilongjiang University of Chinese Medicine  Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, First Affiliated Hospital Heilongjiang University of Chinese Medicine  Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong\u2010Li","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, First Affiliated Hospital Heilongjiang University of Chinese Medicine  Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Duo\u2010Jia","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, First Affiliated Hospital Heilongjiang University of Chinese Medicine  Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, First Affiliated Hospital Heilongjiang University of Chinese Medicine  Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui\u2010Zhe","family":"Zhang","sequence":"additional","affiliation":[{"name":"Center for Reproductive Medicine, Henan Key Laboratory of Reproduction and Genetics The First Affiliated Hospital of Zhengzhou University  Zhengzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard K. T.","family":"Kam","sequence":"additional","affiliation":[{"name":"Department of Chemical Pathology Prince of Wales Hospital  Hong Kong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chung Shun","family":"Ho","sequence":"additional","affiliation":[{"name":"Department of Chemical Pathology Prince of Wales Hospital  Hong Kong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael Ho Ming","family":"Chan","sequence":"additional","affiliation":[{"name":"Department of Chemical Pathology Prince of Wales Hospital  Hong Kong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronald Ching Wan","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Medicine and Therapeutics The Chinese University of Hong Kong  Hong Kong China"},{"name":"Li Ka Shing Institute of Health Sciences and School of Biomedical Sciences The Chinese University of Hong Kong  Hong Kong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ernest Hung Yu","family":"Ng","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology The University of Hong Kong  Hong Kong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ben Willem J.","family":"Mol","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology, Monash Medical Centre Monash University  Clayton Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3424-1502","authenticated-orcid":false,"given":"Elisabet","family":"Stener\u2010Victorin","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pharmacology Karolinska Institutet  Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard S.","family":"Legro","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Pennsylvania State University  Hershey Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3928-7278","authenticated-orcid":false,"given":"Chi Chiu","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology The Chinese University of Hong Kong  Hong Kong China"},{"name":"Li Ka Shing Institute of Health Sciences and School of Biomedical Sciences The Chinese University of Hong Kong  Hong Kong China"},{"name":"Chinese University of Hong Kong\u2010Sichuan University Joint Laboratory in Reproductive Medicine The Chinese University of Hong Kong  Hong Kong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1767-7344","authenticated-orcid":false,"given":"Xiao\u2010Ke","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, First Affiliated Hospital Heilongjiang University of Chinese Medicine  Harbin China"},{"name":"The First Affiliated Hospital of Zhejiang Chinese Medical University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"PCOSAct Study Group","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,1,28]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2023.07.025"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrendo.2018.24"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/det262"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1210\/er.2015\u20101104"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dmaa051"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591\u2010019\u20100666\u20101"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcna.2021.06.008"},{"key":"e_1_2_11_9_1","first-page":"59","volume-title":"Adv Clin Chem","author":"Shea J. 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