{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T10:51:11Z","timestamp":1791802271044,"version":"4.3.4"},"reference-count":28,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T00:00:00Z","timestamp":1770940800000},"content-version":"vor","delay-in-days":43,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100014188","name":"Ministry of Science and ICT, South Korea","doi-asserted-by":"publisher","award":["RS\u20102023\u201000221200"],"award-info":[{"award-number":["RS\u20102023\u201000221200"]}],"id":[{"id":"10.13039\/501100014188","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Medicine &amp; Biology"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Purpose<\/jats:title>\n                    <jats:p>To evaluate whether in\u00a0vitro caffeine supplementation can improve spindle integrity, embryo development, and clinical outcomes in IVF.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>This retrospective paired\u2010cohort study included 704 women aged \u2265\u200935\u2009years who underwent two ICSI cycles (one caffeine\u2010supplemented and one non\u2010caffeine) within 12\u2009months. Oocytes in caffeine cycles were exposed to 1.25\u2009mM caffeine for 4\u2009h before fertilization. Outcomes included spindle positioning, embryo quality, blastocyst development and euploidy rates, and neonatal outcomes from 20 women (24 neonates). Paired comparisons were performed using the Wilcoxon signed\u2010rank and McNemar's tests.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Caffeine cycles showed higher rates of normal spindle positioning (80.2% vs. 61.2%,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001), high\u2010quality cleavage embryos (63.2% vs. 53.6%,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001), high\u2010quality blastocysts (39.2% vs. 26.1%,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001), and euploid embryos (22.9% vs. 10.9%,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.002) than non\u2010caffeine cycles. The fertilization rates were similar between the groups (81.4% vs. 80.8%,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.628). Clinical pregnancy rate was 39.1%, and neonatal outcomes were reassuring.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Short\u2010term in\u00a0vitro caffeine exposure may enhance oocyte and embryo competence in older women undergoing IVF, especially those with prior IVF failures. These findings support caffeine as a simple, practical laboratory intervention. Prospective multicenter studies are warranted to confirm efficacy and safety.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rmb2.70029","type":"journal-article","created":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T11:18:19Z","timestamp":1770981499000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Caffeine Supplementation Enhances Aged Human Oocyte Quality and Embryo Development in In\u00a0Vitro Fertilization: A Retrospective Paired Study"],"prefix":"10.1002","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8168-2968","authenticated-orcid":false,"given":"Jinkyeong","family":"Ha","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology CHA University Fertility Center Gangnam  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soyoung","family":"Kim","sequence":"additional","affiliation":[{"name":"IVF and Fertility Research Lab CHA University Fertility Center Gangnam  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyunmee","family":"Jang","sequence":"additional","affiliation":[{"name":"IVF and Fertility Research Lab CHA University Fertility Center Gangnam  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoo Ra","family":"Ko","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology CHA University Fertility Center Gangnam  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seyul","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology CHA University Fertility Center Gangnam  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Woo Sik","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology CHA University Fertility Center Gangnam  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin Hee","family":"Eum","sequence":"additional","affiliation":[{"name":"IVF and Fertility Research Lab CHA University Fertility Center Gangnam  Seoul Republic of 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