{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T09:48:45Z","timestamp":1791798525954,"version":"4.3.4"},"reference-count":71,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T00:00:00Z","timestamp":1778457600000},"content-version":"vor","delay-in-days":130,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Medicine &amp;amp; Biology"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Acute scrotal hyperthermia is a recognized spermatogenesis stressor, but recovery of spermatogonial stem cells after a single mild heat exposure remains unclear, particularly over cycle\u2010relevant timeframes. This systematic review evaluates the in\u00a0vivo local scrotal hyperthermia across at least one full spermatogenic cycle.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A PICO\u2010driven search identified 18 mouse and rat studies using a single scrotal heating exposure. Outcomes were synthesized thematically across structural, cellular, endocrine\/apoptotic, and sperm domains.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Injury patterns were graded and temperature\u2010 and species\u2010dependent, with more consistent abnormalities at 43\u00b0C and milder effects in rats than in mice. Structural endpoints often remained abnormal at the longest follow\u2010up, and architectural repair did not reliably achieve functional recovery. Mouse stereology at 43\u00b0C indicated long\u2010term SSC and germ\u2010cell depletion, with Leydig\u2010cell and partial Sertoli\u2010cell losses; rat stereology was sparse and absent at 42\u00b0C. Testosterone responses were heterogeneous, whereas tubular apoptosis was more consistently elevated in mice at 43\u00b0C. Sperm concentration\/count declined where assessed, with more frequent impairments of motility and other quality indices, including DNA integrity, at 43\u00b0C.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Recovery was often incomplete and endpoint\u2010specific, with impairment more consistently detected at 43\u00b0C, particularly in mice. Future studies require standardized thermal dosing, transparent bias\u2010mitigation, and SSC\/niche\u2010resolved functional assays.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rmb2.70055","type":"journal-article","created":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T04:30:29Z","timestamp":1778560229000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Reversibility of Spermatogonial Stem Cell Injury After Single Acute Scrotal Hyperthermia in Mouse and Rat Models: A Systematic Review"],"prefix":"10.1002","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9933-9837","authenticated-orcid":false,"given":"Rashid A.","family":"Aldahhan","sequence":"first","affiliation":[{"name":"Department of Anatomy, College of Medicine Imam Abdulrahman Bin Faisal University  Dammam Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,11]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/biolre\/ioy077"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2022.907989"},{"key":"e_1_2_9_4_1","volume-title":"StemBook: The Stem Cell Research Community","author":"Zhou Q.","year":"2008"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.146571"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00013.2015"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2021.111216"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-443-21477-6.00419-3"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/brv.12921"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1001150307"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0220205"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2019.110546"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1530\/rep.0.1220255"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1530\/rep.0.1240133"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod66.5.1374"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.108.071779"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s43032-022-01133-4"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-019-1335-5"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.204239"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.3109\/01485018708986808"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0077351"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2011.10.170"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.n71"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.3109\/15368378.2013.795156"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmjebm-2022-112185"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jclinepi.2005.11.010"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.2147\/IJWH.S309299"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dmac043"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1136\/svn-2024-003235"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.21315\/mjms2024.31.4.3"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41398-022-01967-1"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.l6890"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2020.117767"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tice.2012.04.009"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/and.13664"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.regpep.2010.12.002"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.4149\/BLL_2015_051"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.9734\/JPRI\/2018\/42177"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.acthis.2020.151632"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.acthis.2022.151949"},{"key":"e_1_2_9_41_1","first-page":"45","article-title":"Effect of Pentoxifylline on the Regeneration of Rat Testicular Germ Cells After Heat Shock","volume":"10","author":"Queiroz G. 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