{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T19:37:43Z","timestamp":1792611463721,"version":"4.5.0"},"reference-count":54,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T00:00:00Z","timestamp":1781740800000},"content-version":"vor","delay-in-days":168,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Medicine & Biology"],"published-print":{"date-parts":[[2026,1]]},"abstract":"ABSTRACT<\/jats:title>\n \n Purpose<\/jats:title>\n Leukemia Inhibitory Factor (LIF) plays a crucial role in implantation and early pregnancy maintenance. However, its regulatory relationships with molecular markers such as Sirtuin 1 (SIRT1), immunoglobulin\u2010like transcript 4 (ILT\u20104), and specific microRNAs (miRNA) in recurrent pregnancy loss (RPL) remain underexplored.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n This study investigates the effect of LIF on the expression of\n Sirt1<\/jats:italic>\n ,\n Ilt\u20104<\/jats:italic>\n , and related miRNAs using a mouse model of RPL. Female CBA\/J mice mated with DBA\/2\u2009J males received LIF treatment. Gene and miRNA expression levels were assessed via quantitative PCR.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n The LIF\u2010treated group showed significant upregulation of\n Sirt1<\/jats:italic>\n on Days 7 and 14.\n Ilt\u20104<\/jats:italic>\n expression also increased significantly on Days 7 and 14 in the LIF group. Among miRNAs,\n mir\u2010138\u20105p<\/jats:italic>\n was significantly upregulated on Day 14 (\n p<\/jats:italic>\n \u2009=\u20090.0001), while\n mir\u2010223\u20103p<\/jats:italic>\n was downregulated on Days 7 (\n p<\/jats:italic>\n \u2009=\u20090.02) and 14 (\n p<\/jats:italic>\n \u2009=\u20090.001).\n mir\u2010199a\u20105p<\/jats:italic>\n showed significant downregulation on Days 7 (\n p<\/jats:italic>\n \u2009<\u20090.0001) and 14 (\n p<\/jats:italic>\n \u2009=\u20090.0001). Additionally,\n mir\u2010155\u20105p<\/jats:italic>\n demonstrated a significant decrease in the LIF\u2010treated group compared to the PBS group (\n p<\/jats:italic>\n \u2009<\u20090.0001).\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n These results suggest that LIF modulates critical molecular pathways in RPL by influencing the expression of essential genes and miRNAs, highlighting its potential as a therapeutic target for pregnancy\u2010related complications.<\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/rmb2.70062","type":"journal-article","created":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T05:38:14Z","timestamp":1781847494000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The Impact of Leukemia Inhibitory Factor on\n Sirtuin1<\/i>\n ,\n Ilt\u20104<\/i>\n , and Related\n microRNAs<\/scp>\n Expression in a Mouse Model of Recurrent Pregnancy Loss"],"prefix":"10.1002","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0340-5455","authenticated-orcid":false,"given":"Seyed Mohammad","family":"Seifati","sequence":"first","affiliation":[{"name":"Reproductive Immunology Research Center Shahid Sadoughi University of Medical Sciences Yazd Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0436-9924","authenticated-orcid":false,"given":"Fateme","family":"Zare","sequence":"additional","affiliation":[{"name":"Reproductive Immunology Research Center Shahid Sadoughi University of Medical Sciences Yazd Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5731-4612","authenticated-orcid":false,"given":"Hossein","family":"Ansariniya","sequence":"additional","affiliation":[{"name":"Reproductive Immunology Research Center Shahid Sadoughi University of Medical Sciences Yazd Iran"},{"name":"Department of Immunology Shahid Sadoughi University of Medical Sciences Yazd Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3743-4449","authenticated-orcid":false,"given":"Farzaneh","family":"Fesahat","sequence":"additional","affiliation":[{"name":"Reproductive Immunology Research Center Shahid Sadoughi University of Medical Sciences Yazd Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samaneh","family":"Harimi","sequence":"additional","affiliation":[{"name":"Reproductive Immunology Research Center Shahid Sadoughi University of Medical Sciences Yazd Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maryam","family":"Imani","sequence":"additional","affiliation":[{"name":"Reproductive Immunology Research Center Shahid Sadoughi University of Medical Sciences Yazd Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hossein","family":"Hadinedoushan","sequence":"additional","affiliation":[{"name":"Reproductive 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