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In the present study, we examined the effects of 5\u2010HT receptor agonists on motility, hyperactivation, motility kinematics, and IVF success in mice.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Spermatozoa were incubated for 4\u2009h at 37\u00b0C under 5% CO\n                      <jats:sub>2<\/jats:sub>\n                      in modified Tyrode's albumin lactate pyruvate medium to induce hyperactivation. Motility and hyperactivation were analyzed using a video microscope. Motility kinematics were evaluated using the Sperm Motility Analysis System. IVF was performed by co\u2010incubation of spermatozoa and oocytes for 0.5 and 5\u2009h.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      5\u2010HT receptors were detected. 5\u2010HT\n                      <jats:sub>2<\/jats:sub>\n                      , 5\u2010HT\n                      <jats:sub>2A<\/jats:sub>\n                      , 5\u2010HT\n                      <jats:sub>2B<\/jats:sub>\n                      , and 5\u2010HT\n                      <jats:sub>4<\/jats:sub>\n                      receptor agonists significantly enhanced hyperactivation and increased IVF success. 5\u2010HT\n                      <jats:sub>3<\/jats:sub>\n                      and 5\u2010HT\n                      <jats:sub>7<\/jats:sub>\n                      receptor agonists slightly enhanced hyperactivation but did not affect IVF success. These agonists did not affect motility. During hyperactivation, the velocities of motility kinematics decreased. 5\u2010HT\n                      <jats:sub>2<\/jats:sub>\n                      , 5\u2010HT\n                      <jats:sub>2A<\/jats:sub>\n                      , 5\u2010HT\n                      <jats:sub>2B<\/jats:sub>\n                      , and 5\u2010HT\n                      <jats:sub>4<\/jats:sub>\n                      receptor agonists accelerated the decrease in velocities.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      These results suggest that 5\u2010HT\n                      <jats:sub>2<\/jats:sub>\n                      and 5\u2010HT\n                      <jats:sub>4<\/jats:sub>\n                      receptors participate in the enhancement of hyperactivation and the increase in IVF success in mice. The influence of the 5\u2010HT\n                      <jats:sub>4<\/jats:sub>\n                      receptor agonist is stronger than that of the 5\u2010HT\n                      <jats:sub>2<\/jats:sub>\n                      receptor agonists.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rmb2.70092","type":"journal-article","created":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T06:51:11Z","timestamp":1789627871000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Influences of 5\u2010Hydroxytryptamine Receptor Agonists on Spermatozoal Hyperactivation and In\u00a0Vitro Fertilization Success in Mice"],"prefix":"10.1002","volume":"25","author":[{"given":"Gaku","family":"Nakamura","sequence":"first","affiliation":[{"name":"Department of Urology Dokkyo Medical University Mibu Tochigi 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