{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T08:33:56Z","timestamp":1793003636438,"version":"4.5.0"},"reference-count":39,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T00:00:00Z","timestamp":1791417600000},"content-version":"vor","delay-in-days":280,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Medicine &amp; Biology"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Purpose<\/jats:title>\n                    <jats:p>\n                      Cabergoline, a dopamine D\n                      <jats:sub>2<\/jats:sub>\n                      receptor agonist widely used to prevent ovarian hyperstimulation syndrome (OHSS), has unclear effects on granulosa cells during the follicular phase. This study aimed to investigate how cabergoline affects follicle\u2010stimulating hormone (FSH)\u2010induced cell differentiation and steroidogenesis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Primary granulosa cells were isolated from immature female rats pretreated with diethylstilbestrol (DES) and cultured under four conditions: control, cabergoline alone, FSH alone, and FSH combined with cabergoline. mRNA expression of\n                      <jats:italic>Lhcgr<\/jats:italic>\n                      ,\n                      <jats:italic>Cyp19a1<\/jats:italic>\n                      , and related genes was quantified by real\u2010time PCR, and luteinizing hormone (LH) receptor binding, estradiol (E\n                      <jats:sub>2<\/jats:sub>\n                      ) and progesterone (P\n                      <jats:sub>4<\/jats:sub>\n                      ) secretion, and cyclic adenosine monophosphate (cAMP) production were evaluated. Dopamine D\n                      <jats:sub>2<\/jats:sub>\n                      receptor expression was analyzed by Western blotting.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Cabergoline significantly suppressed FSH\u2010induced mRNA expression of\n                      <jats:italic>Lhcgr<\/jats:italic>\n                      ,\n                      <jats:italic>Cyp19a1<\/jats:italic>\n                      ,\n                      <jats:italic>Cyp11a1<\/jats:italic>\n                      , and\n                      <jats:italic>Hsd3b1<\/jats:italic>\n                      ; hCG binding; E\n                      <jats:sub>2<\/jats:sub>\n                      and P\n                      <jats:sub>4<\/jats:sub>\n                      secretion; and cAMP accumulation. Most inhibitory effects were dose dependent, although cAMP suppression did not exhibit clear concentration dependency. Dopamine D\n                      <jats:sub>2<\/jats:sub>\n                      receptor expression was detectable, although at low levels.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      Cabergoline suppresses FSH\u2010induced granulosa cell differentiation and steroidogenesis, at least partly through attenuation of FSH\u2010induced cAMP signaling. Although dopamine D\n                      <jats:sub>2<\/jats:sub>\n                      receptor signaling may contribute to the inhibitory effects of cabergoline, the downstream molecular mechanisms remain unclear, warranting further investigation.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rmb2.70104","type":"journal-article","created":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T08:47:30Z","timestamp":1791449250000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Cabergoline Suppresses\n                    <scp>FSH<\/scp>\n                    \u2010Induced Differentiation and Steroidogenesis in Primary Cultured Rat Granulosa Cells"],"prefix":"10.1002","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-3333-001X","authenticated-orcid":false,"given":"Yusuke","family":"Mori","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology The Jikei University School of Medicine  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1562-410X","authenticated-orcid":false,"given":"Hiroshi","family":"Kishi","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology The Jikei University School of Medicine  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayako","family":"Hasunuma","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology The Jikei University School of Medicine  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6369-1086","authenticated-orcid":false,"given":"Yuta","family":"Kasahara","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology The Jikei University School of Medicine  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5079-0464","authenticated-orcid":false,"given":"Aikou","family":"Okamoto","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology The Jikei University School of Medicine  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,8]]},"reference":[{"key":"e_1_2_10_2_1","first-page":"202","article-title":"Cabergoline in the Treatment of Parkinson's Disease","volume":"18","author":"Pastor P.","year":"2003","journal-title":"Neurolog\u00eda"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrendo.2011.37"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2007-0409"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2013.11.005"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2006-0657"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2014.01.031"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2014.07.1240"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1080\/07435809809135528"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-90-6-1492"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/279336a0"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1210\/edrv-5-1-76"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-111-4-1192"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.140.11.7112"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1530\/JOE-22-0254"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00432.2012"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11033-019-04755-1"},{"key":"e_1_2_10_19_1","article-title":"Inhibition of Follicle\u2010Stimulating Hormone\u2010Induced Preovulatory Follicles in Rats Treated With a Nonsteroidal Negative Allosteric Modulator of Follicle\u2010Stimulating Hormone Receptor","volume":"90","author":"Dias J. 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