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Because of the lack of an efficient cure for chronic hepatitis B, the main goal of antiviral therapy is the prevention of liver disease progression coupled with prolonged survival of patients. Because HBV viral load has been shown to be a crucial determinant of the progression of liver damage, these goals can be achieved as long as HBV replication can be suppressed. Unfortunately, long\u2010term therapy with the low\u2010to\u2010moderate genetic barrier drugs, which are still recommended in a majority of developing countries, are strongly associated with HBV resistance development and treatment failure. In such cases, the precise and accurate determination of drug\u2010resistant variants in an individual patient before treatment is important for a proper choice of first\u2010line potent therapy. Nowadays, a number of techniques are available to study HBV quasispecies evolution. This review describes the advantages and limitations of various assays detecting drug\u2010resistant HBV variants. Copyright \u00a9 2016 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/rmv.1897","type":"journal-article","created":{"date-parts":[[2016,8,10]],"date-time":"2016-08-10T03:53:48Z","timestamp":1470801228000},"page":"369-381","source":"Crossref","is-referenced-by-count":11,"title":["Current molecular methods for the detection of hepatitis B virus quasispecies"],"prefix":"10.1002","volume":"26","author":[{"given":"Magda","family":"Rybicka","sequence":"first","affiliation":[{"name":"Intercollegiate Faculty of Biotechnology University of Gdansk and Medical University of Gdansk  Gdansk Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Piotr","family":"Stalke","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases Medical University of Gdansk  Gdansk Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krzysztof Piotr","family":"Bielawski","sequence":"additional","affiliation":[{"name":"Intercollegiate Faculty of 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