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It infects recurrently throughout human life without causing severe disease. Because hPIV2 has a viral envelope that can carry ectopic proteins, we developed a non\u2010propagative RNA\/protein\u2010carrying vector BC\u2010PIV by deleting the <jats:italic>F<\/jats:italic> gene from hPIV2. BC\u2010PIV can be vigorously proliferated in the stable packaging cell line Vero\/BC\u2010F cells expressing the hPIV2 <jats:italic>F<\/jats:italic> gene but not in other cells. BC\u2010PIV can deliver exogenous gene(s) on a multigenic RNA genome as an inserted gene fragment(s) and simultaneously deliver exogenous protein(s) on its envelope in a membrane\u2010anchored form. For example, influenza virus M2e protein, Ebola virus GP protein, and severe acute respiratory syndrome\u2010coronavirus\u20102 (SARS\u2010CoV\u20102) spike protein were shown to be highly expressed in packaging cells and incorporated into the virion. The Ebola virus GP protein and SARS\u2010CoV\u20102 spike protein, each delivered via BC\u2010PIV, efficiently induced neutralising antibodies against each virus, even after prior treatment with recombinant BC\u2010PIV in mice and hamsters, respectively. In this review, we describe the properties of BC\u2010PIV as a promising vaccine vector, and also demonstrate its application as an anti\u2010tumour virus.<\/jats:p>","DOI":"10.1002\/rmv.70008","type":"journal-article","created":{"date-parts":[[2024,11,3]],"date-time":"2024-11-03T04:29:11Z","timestamp":1730608151000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Next Generation RNA\/Protein\u2010Carrying Vector With Pleiotropic Activity"],"prefix":"10.1002","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0037-7049","authenticated-orcid":false,"given":"Tetsuya","family":"Nosaka","sequence":"first","affiliation":[{"name":"Department of Microbiology and Molecular Genetics Mie University Graduate School of Medicine  Tsu Japan"},{"name":"Research Centre for Development of Recombinant VLP Vaccines Research Institutes of Excellence Mie University  Tsu Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junpei","family":"Ohtsuka","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Molecular Genetics Mie University Graduate School of Medicine  Tsu Japan"},{"name":"Research Centre for Development of Recombinant VLP Vaccines Research Institutes of Excellence Mie University  Tsu Japan"},{"name":"BioComo Inc.  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