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Our study uses a reverse vaccinology strategy to design a vaccine containing the viral Major capsid protein VP1 protein of the Simian virus's B and T\u2010cell epitopes. Eleven MHC\u2010I restricted, ten MHC\u2010II restricted, and five B\u2010cell specific epitopes were prioritised for the design of vaccine model, based on non\u2010allergenicity as a crucial feature and an antigenicity score of &gt;\u00a00.4. An immunogenic and stable vaccine design was generated using the 6\u2010histidine tag, including a 30S ribosomal protein AS04 as an adjuvant with linkers (EAAAK, GPGPG, and AYY). Highly broad\u2010spectrum vaccine was generated with 99.75% of global population coverage. Ramachandran score of 91.7% indicated the structural stability of the designed vaccine. The immunological simulations presented that a persistent antibody response occurred even if the antigen was expelled. IgM\u00a0+\u00a0IgG titres were predicted to rise to 6000 after 10\u00a0days of injection, and stabilised at 3000 after 30\u00a0days, suggesting that the vaccine is effective and provides long\u2010lasting protection against SV40. Molecular docking and MD simulation analyses were performed to study the stability and dynamic confirmation of the designed vaccine model and immune receptor (8JBV), validating strong molecular interactions, hence triggering innate and immune responses against the SV40. Further immunological assays are required to validate the results of this study.<\/jats:p>","DOI":"10.1002\/rmv.70065","type":"journal-article","created":{"date-parts":[[2025,8,9]],"date-time":"2025-08-09T03:59:43Z","timestamp":1754711983000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Immunoinformatics\u2010Driven Design of a Multi\u2010Epitope Vaccine Targeting Simian Virus VP1 Major Capsid Protein for Oncogenic Viral Infection Prevention"],"prefix":"10.1002","volume":"35","author":[{"given":"Roman","family":"Akbar","sequence":"first","affiliation":[{"name":"Department of Computer Science and Bioinformatics Khushal Khan Khattak University  Karak Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shabeen","family":"Fatima","sequence":"additional","affiliation":[{"name":"Institute of Inorganic Chemistry Slovak Academy of Sciences  Bratislava Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siddiq ur","family":"Rahman","sequence":"additional","affiliation":[{"name":"Department of Computer Science and Bioinformatics Khushal Khan Khattak University  Karak Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdul","family":"Malik","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics College of Pharmacy King Saud University  Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suhail","family":"Akhtar","sequence":"additional","affiliation":[{"name":"A.T. 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