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Several different methods have been used to generate SARS\u2010CoV specific neutralizing hmAbs including the immunization of transgenic mice, cloning of small chain variable regions from na\u00efve and convalescent patients, and the immortalization of convalescent B cells. Irrespective of the techniques used, the majority of hmAbs specifically reacted with the receptor binding domain (RBD) of the spike (S) protein and likely prevented receptor binding. However, several hmAbs that can bind to epitopes either within the RBD, located N terminal of the RBD or in the S2 domain, and neutralize the virus with or without inhibiting receptor binding have been identified. Therapeutic utility of hmAbs has been further elucidated through the identification of potential combinations of hmAbs that could neutralize viral variants including escape mutants selected using hmAbs. These results suggest that a cocktail of hmAbs that can bind to unique epitopes and have different mechanisms of action might be of clinical utility against SARS\u2010CoV infection, and indicate that a similar approach may be applied to treat other viral infections. Copyright \u00a9 2011 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/rmv.706","type":"journal-article","created":{"date-parts":[[2011,9,8]],"date-time":"2011-09-08T13:44:50Z","timestamp":1315489490000},"page":"2-17","source":"Crossref","is-referenced-by-count":73,"title":["Neutralizing human monoclonal antibodies to severe acute respiratory syndrome coronavirus: target, mechanism of action, and therapeutic potential"],"prefix":"10.1002","volume":"22","author":[{"given":"Melissa M.","family":"Coughlin","sequence":"first","affiliation":[{"name":"Centers for Disease Control and Prevention, Measles, Mumps, Rubella and Herpes Virus Laboratory Branch  Atlanta GA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bellur S.","family":"Prabhakar","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology MC790 College of Medicine University of Illinois at Chicago  Chicago IL 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