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The initial elastic response (Young's modulus \u223c 4,000 Pa) of the cells to an applied force was followed by a slow compression of the cytoskeleton (\u03c4<jats:sub>1\/2<\/jats:sub> \u2248 10 s). When force application was terminated, the cytoskeleton underwent a sudden partial decompression and a subsequent slow, incomplete recovery. The role of the cytoskeletal elements in cell mechanics was accessed in AFM measurements carried out on cells treated with cytochalasin D, nocodazole, or col\u2010cemid. Cytochalasin D treatment reduced both elasticity (\u223c45%) and cytoplasmic viscosity (\u223c65%), whereas cells treated with nocodazole or colcemid exhibited a marked increase in elasticity (\u223c100%) and a slight increase in viscosity (\u223c15%). The AFM force measurements also provided evidence that the cell membrane and the cytoskeleton are mechanically coupled. Tightly adherent cells were stiffer than cells that were loosely attached. Moreover, cells crosslinked with either glutaraldehyde, 3,3 \u2018\u2010dithiobis\u2019sul\u2010fosuccinimidylpropionate] (DTSSP), or Concanavalin A were more rigid than untreated cells. It is of interest that cells crosslinked with Concanavalin A, but not DTSSP, displayed plastic behaviors that may reflect the induction of cytoskeletal reorganization by Concanavalin A.<\/jats:p>","DOI":"10.1002\/sca.1998.4950200504","type":"journal-article","created":{"date-parts":[[2010,6,25]],"date-time":"2010-06-25T09:13:23Z","timestamp":1277457203000},"page":"389-397","source":"Crossref","is-referenced-by-count":290,"title":["Mechanical properties of L929 cells measured by atomic force microscopy: Effects of anticytoskeletal drugs and membrane crosslinking"],"prefix":"10.1002","volume":"20","author":[{"given":"H. 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