{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T14:11:58Z","timestamp":1790518318826,"version":"4.1.0"},"reference-count":24,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2010,2,24]],"date-time":"2010-02-24T00:00:00Z","timestamp":1266969600000},"content-version":"vor","delay-in-days":115,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["209760868\/B060805"],"award-info":[{"award-number":["209760868\/B060805"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Scanning"],"published-print":{"date-parts":[[2009,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The poor integration with host cornea tissue and the low mechanical properties of pHEMA hydrogel for artificial cornea remains a difficult problem to solve. A modified pHEMA hydrogel, MMA copolymerized and type\u2010I collagen and bFGF immobilized, was previously prepared in an attempt to solve the problems. In this study, the cytotoxicity of Col\/bFGF\u2010p (HEMA\u2010MMA) and p (HEMA\u2010MMA) was studied by cell adhesion assay and atomic force microscopy (AFM). The results of cell adhesion assay show that the attachment of keratocytes on the modified membrane is much higher than that of the unmodified membrane. This indicates that the material after modification have better cell\u2013material interaction. The AFM images reveal that the morphology of keratocytes cultured on different substrate is obviously different. The cell cultured on modified membrane presented a completely elongated and spindle\u2010shape morphology. The force\u2212distance indicates that the biomechanical of keratocytes changes significantly after culturing on different substrates. The adhesion force (2328\u00b1523\u2009pN) and Young's modulus (0.51\u00b10.125\u2009kPa) of the cell cultured on modified membrane are much higher, and the stiffness (0.08\u00b10.022\u2009mN\/m) is lower than those of the cell cultured on unmodified membrane. These results show that the cytotoxicity of Col\/bFGF\u2010p (HEMA\u2010MMA) for keratocytes is much improved. SCANNING 31: 246\u2013252, 2009. \u00a9 2010 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/sca.20170","type":"journal-article","created":{"date-parts":[[2010,2,24]],"date-time":"2010-02-24T17:33:20Z","timestamp":1267032800000},"page":"246-252","source":"Crossref","is-referenced-by-count":15,"title":["The morphological and biomechanical changes of keratocytes cultured on modified p (HEMA\u2010MMA) hydrogel studied by 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