{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T16:18:59Z","timestamp":1787242739240,"version":"build-2736575974"},"reference-count":13,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2006,12,7]],"date-time":"2006-12-07T00:00:00Z","timestamp":1165449600000},"content-version":"vor","delay-in-days":1985,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Scanning"],"published-print":{"date-parts":[[2001,7]]},"abstract":"Abstract<\/jats:title>\n \n With its output wavelength covering the in\u2010 frared penetrating window of most biological tissues at 1200\u20131250 nm, the femtosecond Cr:forsterite laser shows high potential to serve as an excellent excitation source for the multiphoton fluorescence microscope. Its high output power, short optical pulse width, high stability, and low dispersion in fibers make it a perfect replacement for the currently widely used Ti: sapphire laser. In this paper, we study the capability of using a femtosecond Cr: forsterite laser in multiphoton scanning microscopy. We have performed the multiphoton excited photolumines\u2010cence spectrum measurement on several commonly used bioprobes using the 1230 nm femtosecond pulses from a Cr:forsterite laser. Efficient fluorescence can be easily observed in these bioprobes through two\u2010photon or three\u2010photon excitation processes. These results will assist in the selection of dichroic beam splitter and band pass filters in a multiphoton microscopic system. We have also performed the autofluorescence spectrum measurement from chlorophylls in live leaves of the plant\n Arabidopsis thaliana<\/jats:italic>\n excited by 1230 nm femtosecond pulses from the Cr:forsterite laser. Bright luminescence from chlorophyll, centered at 673 and 728 nm, respectively, can be easily observed. Taking advantage of the bright two\u2010photon photoluminescence from chlorophyll, we demonstrated the two\u2010photon scanning paradermal and cross\u2010sectional images of palisade mesophyll cells in live leaves of\n Arabidopsis thaliana<\/jats:italic>\n .\n <\/jats:p>","DOI":"10.1002\/sca.4950230405","type":"journal-article","created":{"date-parts":[[2010,6,24]],"date-time":"2010-06-24T13:33:11Z","timestamp":1277386391000},"page":"249-254","source":"Crossref","is-referenced-by-count":42,"title":["Multiphoton confocal microscopy using a femtosecond Cr:Forsterite laser"],"prefix":"10.1002","volume":"23","author":[{"given":"Tzu\u2010Ming","family":"Liu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shi\u2010Wei","family":"Chu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chi\u2010Kuang","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bai\u2010Ling","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ping\u2010Chin","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"Johnson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,12,7]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12479191"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2818.1998.00277.x"},{"key":"e_1_2_1_4_1","doi-asserted-by":"crossref","unstructured":"ChudobaC FujimotoJG IppenEP HausHA MorgnerU K\u00e4rt\u2010nerFX ScheurerV AngelowG TschudiT:All\u2010solid\u2010state Cr:forsterite laser generating 14\u2010fs pulses at 1.3 \u03bcm.CLEO 2000 Postdeadline papers CPD 4\u20131 San Francisco 7\u20138(2000)","DOI":"10.1109\/CLEO.2000.907492"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0320(19970901)29:1<58::AID-CYTO6>3.0.CO;2-9"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2321027"},{"key":"e_1_2_1_7_1","volume-title":"Handbook of Fluorescent Probes and Research Chemicals","author":"Haugland RP","year":"1999"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.271.5254.1420"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1364\/OL.17.000937"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1364\/OL.18.000891"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1109\/3.301649"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1364\/OL.21.000354"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/35048692"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1109\/3.641312"}],"container-title":["Scanning"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fsca.4950230405","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/sca.4950230405","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,19]],"date-time":"2023-11-19T11:21:46Z","timestamp":1700392906000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/sca.4950230405"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,7]]},"references-count":13,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2001,7]]}},"alternative-id":["10.1002\/sca.4950230405"],"URL":"https:\/\/doi.org\/10.1002\/sca.4950230405","archive":["Portico"],"relation":{},"ISSN":["0161-0457","1932-8745"],"issn-type":[{"value":"0161-0457","type":"print"},{"value":"1932-8745","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,7]]}}}